Sharma Priya, Kumar Vineet, Khosla Rajiv, Guleria Praveen
Plant Biotechnology and Genetic Engineering Lab, Department of Biotechnology, DAV University, Jalandhar, Punjab 144012 India.
Department of Biotechnology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab 144111 India.
3 Biotech. 2020 Oct;10(10):431. doi: 10.1007/s13205-020-02428-6. Epub 2020 Sep 15.
Flavonoid exposure is known to affect the antioxidant profile of legumes. However, a detailed study evaluating the effect of flavonoid naringenin on morphology and biochemical profile of legume is lacking. The present study is a novel report of improved protein bioavailability and antioxidant potential of legume mungbean on naringenin exposure. The quantitative evaluation revealed significant protein accumulation (64-122 μg/g FW) on naringenin exposure. Further, an increase in protein solubility and digestibility compared to control was evident. Naringenin mediated altered α-amylase activity improved the mungbean seed germination rate. Naringenin induced auxin redistribution and altered PIN formed transcript expression reduced lateral root density and increased stem length that was subsequently reverted on exogenous indole acetic acid application. Naringenin enhanced polyphenolic accumulation and improved the antioxidant potential of mungbean. Additionally, the responsiveness of the early gene of the flavonoid biosynthetic pathway, to naringenin concentration was revealed indicating a probable feedback regulation. Further, the presence of alternate liquiritigenin biosynthesis was also evident. The present study, thus reveals the probable potential of phytochemical naringenin towards agricultural sustainability in the changing environmental conditions.
已知黄酮类化合物的暴露会影响豆类的抗氧化特性。然而，缺乏一项详细研究来评估黄酮类化合物柚皮素对豆类形态和生化特性的影响。本研究首次报道了柚皮素暴露可提高豆类绿豆的蛋白质生物利用度和抗氧化潜力。定量评估显示，柚皮素暴露后蛋白质显著积累（64 - 122μg/g鲜重）。此外，与对照相比，蛋白质溶解度和消化率明显增加。柚皮素介导的α -淀粉酶活性改变提高了绿豆种子发芽率。柚皮素诱导生长素重新分布，改变PIN形成转录本表达，降低侧根密度并增加茎长，随后在外源吲哚乙酸处理后恢复。柚皮素增强了多酚积累，提高了绿豆的抗氧化潜力。此外，还揭示了黄酮类生物合成途径早期基因对柚皮素浓度的响应，表明可能存在反馈调节。此外，也明显存在异甘草素的替代生物合成。因此，本研究揭示了植物化学物质柚皮素在不断变化的环境条件下对农业可持续性的潜在可能性。
