Sección de Fisiologia Vegetal, Facultad de Farmacia, Universidad de Barcelona, E-08028 Barcelona, Spain.
Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CSIC), 28040 Madrid, Spain.
J Plant Physiol. 2018 Sep;228:47-58. doi: 10.1016/j.jplph.2018.05.007. Epub 2018 May 18.
The anticancer compound podophyllotoxin and other related lignans can be produced in Linum album in vitro cultures, although their biosynthesis varies according to the degree of differentiation of the plant material. In general, L. album cell cultures do not form the same lignans as roots or other culture systems. Our aim was to explore how the lignan-producing capacity of organogenic cell masses is affected by the conditions that promote their formation and growth. Thus, L. album biomass obtained from plantlets was cultured in darkness or light, with or without the addition of plant growth regulators, and the levels of podophyllotoxin, methoxypodophyllotoxin and other related lignans were determined in each of these conditions. The organogenic capacity of the cell biomass grown in the different conditions was studied directly and also with light and scanning electronic microscopy, leading to the observation of.several somatic embryos and well-formed shoots. The main lignan produced was methoxypodophyllotoxin, whose production was clearly linked to the organogenic capacity of the cell biomass, which to a lesser extent was also the case for podophyllotoxin.
抗癌化合物鬼臼毒素和其他相关木脂素可以在亚麻体外培养物中产生，尽管它们的生物合成根据植物材料的分化程度而有所不同。一般来说，亚麻细胞培养物不会形成与根或其他培养系统相同的木脂素。我们的目的是探讨促进其形成和生长的条件如何影响产木质素的器官发生细胞团的产木质素能力。因此，从幼苗获得的亚麻生物量在黑暗或光照下培养，有或没有添加植物生长调节剂，并在每种条件下测定鬼臼毒素、甲氧基鬼臼毒素和其他相关木脂素的水平。用直接和光镜及扫描电子显微镜研究了在不同条件下生长的细胞生物质的器官发生能力，导致观察到几个体细胞胚和形态良好的芽。主要产生的木质素是甲氧基鬼臼毒素，其产生明显与细胞生物质的器官发生能力有关，而鬼臼毒素的情况则较少。
