Redox Biology Laboratory, Department of Zoology, School of Life Sciences, Ravenshaw University, Cuttack, 753003, Odisha, India.
Redox Biology Laboratory, Department of Zoology, School of Life Sciences, Ravenshaw University, Cuttack, 753003, Odisha, India.
Environ Toxicol Pharmacol. 2018 Jul;61:71-78. doi: 10.1016/j.etap.2018.05.007. Epub 2018 May 22.
Iron though an essential cofactor for many proteins including haemoglobin and cytochromes, when in excess (>1 ppm in water and 100 ppm in fish tissue) elicits toxicity via Fenton reaction inducing oxidative stress. The present study aimed to evaluate the efficacy of dietary Spirulina (Arthrospira platensis) supplementation on waterborne-iron induced oxidative stress in the tissues of Notopterus notopterus. Juvenile fishes were divided randomly into 4 groups, namely, Group-I: control fed with commercial diet only, Group-II, III and IV treated with 0.75 ppm FeCl where Group-II fed with commercial diet only, Group-III with 10% (w/w) Spirulina supplemented commercial diet and Group-IV with 100% (w/w) Spirulina diet only; for 7 and 28 days (n = 6 per group). Tissue oxidative stress biomarkers like lipid peroxidation (LPx), protein carbonylation (PC) and protein thionylation (protein and nonprotein-SH content); antioxidant defence (superoxide dismutase: SOD; catalase; CAT; glutathione peroxidase/reductase: GPx/GR; glutathione s-transferase: GST; metalothionine: MT and reduced glutathione: GSH) and iron accumulation in the gill, liver and muscles tissue were analysed. The augmented oxidative predominance in the tissues with respect to LPx and PC along with decline in antioxidant defence (SOD, CAT, GPx, GR, GST, MT, PSH, NPSH and GSH) by iron was neutralized by Spirulina supplementation in the diet in a dose and duration dependent manner where 100% Spirulina diet for 28 days completely ameliorated iron-induced oxidative stress in fish tissues. Thus, Spirulina can be used as a dietary supplement for fishes cultured in water bodies with iron overload.
铁虽然是许多蛋白质的必需辅因子，包括血红蛋白和细胞色素，但当铁过量（水中>1ppm，鱼类组织中>100ppm）时，会通过芬顿反应引发毒性，导致氧化应激。本研究旨在评估膳食螺旋藻（Arthrospira platensis）补充剂对水生铁诱导的 Notopterus notopterus 组织氧化应激的功效。将幼鱼随机分为 4 组，即：第 I 组：仅用商业饲料喂养的对照组；第 II、III 和 IV 组用 0.75ppm FeCl 处理，其中第 II 组仅用商业饲料喂养，第 III 组用 10%（w/w）螺旋藻补充的商业饲料喂养，第 IV 组仅用 100%（w/w）螺旋藻饲料喂养；7 和 28 天（每组 n=6）。分析组织氧化应激生物标志物，如脂质过氧化（LPx）、蛋白质羰基化（PC）和蛋白质硫代化（蛋白质和非蛋白质-SH 含量）；抗氧化防御（超氧化物歧化酶：SOD；过氧化氢酶；CAT；谷胱甘肽过氧化物酶/还原酶：GPx/GR；谷胱甘肽 S-转移酶：GST；金属硫蛋白：MT 和还原型谷胱甘肽：GSH）和铁在鳃、肝和肌肉组织中的积累。铁导致组织中氧化优势增加，LPx 和 PC 增加，抗氧化防御（SOD、CAT、GPx、GR、GST、MT、PSH、NPSH 和 GSH）下降，螺旋藻在饮食中的补充以剂量和时间依赖的方式中和了这一点，其中 28 天的 100%螺旋藻饮食完全缓解了鱼类组织中铁诱导的氧化应激。因此，螺旋藻可用作铁过载水体中养殖鱼类的膳食补充剂。
