He Quan, Liu Qihua, Chen Yongbin, Meng Jiaquan, Zou Ling
Department of Traditional Chinese Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
Evid Based Complement Alternat Med. 2018 May 7;2018:6857398. doi: 10.1155/2018/6857398. eCollection 2018.
Ischemic stroke (IS) is a fatal subtype of stroke that lacks effective treatments. Angiogenesis following IS is an effective response that mediates brain recovery and repair. Our previous study demonstrated that long-zhi decoction (LZD), a Chinese herbal formula, promoted angiogenesis in rats of IS model. To further investigate the association between the proangiogenic mechanism of an LZD-medicated serum and cellular autophagy, we evaluated its promotional effect on angiogenesis in human umbilical vein endothelial cells (HUVECs) in vitro. We used HUVECs subjected to HO to induce injury and observed the effects of the LZD-medicated serum treatment. Cell-based assays included proliferation, migration, and tube formation. To assess the extent of autophagy, transmission electron microscopy was used to measure the number of autophagosomes. Immunofluorescence and Western blotting were performed to evaluate the autophagy-related protein of LC3-II and Beclin-1. The LZD-medicated serum promoted proliferation, migration, and tube formation in HUVECs. The LZD-medicated serum also increased the autophagosomes and the autophagic protein expressions of LC3-II and Beclin-1. The proangiogenic and autophagic activity of LZD provides new cogitations to its clinical application and may lead to potential drug development for treating various vascular diseases, especially in the elderly, in the future.
缺血性中风（IS）是一种缺乏有效治疗方法的致命性中风亚型。IS后的血管生成是介导脑恢复和修复的有效反应。我们之前的研究表明，中药复方龙蛭汤（LZD）可促进IS模型大鼠的血管生成。为进一步研究LZD含药血清促血管生成机制与细胞自噬之间的关联，我们在体外评估了其对人脐静脉内皮细胞（HUVECs）血管生成的促进作用。我们使用经缺氧（HO）诱导损伤的HUVECs，并观察LZD含药血清处理的效果。基于细胞的实验包括增殖、迁移和管腔形成。为评估自噬程度，使用透射电子显微镜测量自噬体数量。进行免疫荧光和蛋白质印迹法以评估自噬相关蛋白LC3-II和Beclin-1。LZD含药血清促进了HUVECs的增殖、迁移和管腔形成。LZD含药血清还增加了自噬体以及LC3-II和Beclin-1的自噬蛋白表达。LZD的促血管生成和自噬活性为其临床应用提供了新的思考，并可能在未来导致治疗各种血管疾病，尤其是老年血管疾病的潜在药物开发。
