Departamento de Biología Molecular, Juan Badiano No. 1, Col. Sección XVI, Delegación Tlalpan, Instituto Nacional de Cardiología "Ignacio Chávez", 14080 Mexico City, Mexico.
Departamento de Biología Celular, Avenida Instituto Politecnico Nacional No. 2508, Col. San Pedro Zacatenco, Centro de Investigación y de Estudios Avanzados del Instituto Politecnico Nacional, 07360 Mexico City, Mexico.
J Immunol Res. 2018 May 7;2018:8964085. doi: 10.1155/2018/8964085. eCollection 2018.
is the protozoan parasite that causes Chagas disease, which is considered by the World Health Organization to be a neglected tropical disease. Two drugs exist for the treatment of Chagas disease, nifurtimox and benznidazole; they are only effective in the acute phase, and a vaccine is currently not available. In this study, we used the recombinant enolase from H8 strain (MHOM/MX/1992/H8 Yucatán) (rTcENO) and its encoding DNA (pBKTcENO) to immunize mice and evaluate their protective effects in an experimental murine model of acute phase infection. Our results showed that mice vaccinated with rTcENO or its encoding DNA were able to generate typical specific antibodies (IgG1, IgG2a, and IgG2b), suggesting that a mixed Th1/Th2 immune response was induced. The parasite burden in the blood was reduced to 69.8% and 71% in mice vaccinated with rTcENO and pBKTcENO, respectively. The group vaccinated with rTcENO achieved 75% survival, in contrast to the group vaccinated with pBKTcENO that showed no survival in comparison to the control groups. Moreover, rTcENO immunization elevated the production of IFN- and IL-2 after the parasite challenge, suggesting that the Th1-type immune response was polarized. These results indicated that rTcENO could be used as a vaccine against Chagas disease.
克氏锥虫是引起恰加斯病的原生动物寄生虫，世界卫生组织认为它是一种被忽视的热带病。目前有两种药物可用于治疗恰加斯病，硝呋莫司和苯并咪唑；它们仅在急性期有效，目前尚无疫苗。在这项研究中，我们使用了来自 H8 株（MHOM/MX/1992/H8 Yucatán）的重组烯醇酶（rTcENO）及其编码 DNA（pBKTcENO）对小鼠进行免疫，并在急性感染的实验性小鼠模型中评估其保护作用。我们的结果表明，用 rTcENO 或其编码 DNA 免疫的小鼠能够产生典型的特异性抗体（IgG1、IgG2a 和 IgG2b），表明诱导了混合 Th1/Th2 免疫反应。用 rTcENO 和 pBKTcENO 免疫的小鼠的血液寄生虫负荷分别降低到 69.8%和 71%。用 rTcENO 免疫的小鼠的存活率达到 75%，而用 pBKTcENO 免疫的小鼠则与对照组相比没有存活。此外，rTcENO 免疫在寄生虫攻击后提高了 IFN-和 IL-2 的产生，表明 Th1 型免疫反应被极化。这些结果表明，rTcENO 可用于预防恰加斯病。
