Departamento de Infectómica y Patogenesis Molecular, Centro de Investigación y de Estudios Avanzados del I.P.N., México D.F. 07360, México.
Int J Biol Sci. 2011;7(9):1230-8. doi: 10.7150/ijbs.7.1230. Epub 2011 Oct 25.
Immunization of mice with plasmids containing genes of Trypanosoma cruzi induces protective immunity in the murine model of Chagas disease. A cDNA clone that codes for an amastigote-specific surface protein (TcSSP4) was used as a candidate to develop a DNA vaccine. Mice were immunized with the recombinant protein rTcSSP4 and with cDNA for TcSSP4, and challenged with bloodstream trypomastigotes. Immunization with rTcSSP4 protein makes mice more susceptible to trypomastigote infection, with high mortality rates, whereas mice immunized with a eukaryotic expression plasmid containing the TcSSP4 cDNA were able to control the acute phase of infection. Heart tissue of gene-vaccinated animals did not show myocarditis and tissue damage at 365 days following infection, as compared with control animals. INF-γ was detected in sera of DNA vaccinated mice shortly after immunization, suggesting the development of a Th1 response. The TcSSP4 gene is a promising candidate for the development of an anti-T. cruzi DNA vaccine.
用含有克氏锥虫基因的质粒免疫小鼠可在恰加斯病的鼠模型中诱导保护性免疫。一种编码无鞭毛体表面蛋白（TcSSP4）的 cDNA 克隆被用作开发 DNA 疫苗的候选物。用重组蛋白 rTcSSP4 和 TcSSP4 的 cDNA 免疫小鼠，并与血源型锥虫感染挑战。用 rTcSSP4 蛋白免疫使小鼠更容易受到锥虫感染，死亡率很高，而用含有 TcSSP4 cDNA 的真核表达质粒免疫的小鼠能够控制感染的急性期。与对照动物相比，在感染后 365 天，基因疫苗接种动物的心脏组织没有心肌炎和组织损伤。在免疫后不久，DNA 疫苗接种小鼠的血清中检测到 IFN-γ，表明产生了 Th1 反应。TcSSP4 基因是开发抗克氏锥虫 DNA 疫苗的有希望的候选物。
