Institute of Food Sciences and Technology, National Taiwan University, Taipei 10617, Taiwan.
Food Funct. 2018 Jun 20;9(6):3363-3373. doi: 10.1039/c7fo02066j.
Polymethoxyflavones (PMFs) and hydroxyl PMFs (HOPMFs) are mainly found in citrus peel and have shown anti-obesity potential in in vitro and in vivo studies. Herein, we have investigated the anti-obesity effects of two citrus peel extracts obtained via supercritical fluid extraction: PMF A, with a lower content of PMFs and HOPMFs, and PMF B, with a higher content of PMFs and HOPMFs. PMF A and PMF B were administered orally for 16 weeks to mice with high fat diet (HFD)-induced obesity. The results showed that PMF B decreased the lipid content more statistically significantly (p < 0.05) than PMF A in 3T3-L1 preadipocytes, reduced the adipocyte size, decreased the adipose tissue weight and alleviated the total body weight in the HFD mice. Both PMF A and PMF B reduced the adipocyte size in the perigonadal fat by markedly decreasing the levels of lipid droplets (LD) and perilipin 1 protein and Sterol regulatory element binding protein 1 (SREBP-1) expression. Compared to the case of the HFD group, PMF B altered the gut microbiota by increasing Prevotella and decreasing rc4-4 bacteria. The change in the composition of gut microbiota, the community of symbiotic and pathogenic microorganisms, may determine the metabolic health and be responsible for the anti-obesity mechanism. Our results indicate that the citrus peel extracts decrease lipid accumulation both in vivo and in vitro and should be considered for the management of overweight and obesity conditions.
多甲氧基黄酮（PMFs）和羟基 PMFs（HOPMFs）主要存在于柑橘皮中，已在体外和体内研究中显示出抗肥胖潜力。在此，我们研究了通过超临界流体萃取获得的两种柑橘皮提取物的抗肥胖作用：PMF A，其 PMFs 和 HOPMFs 含量较低，以及 PMF B，其 PMFs 和 HOPMFs 含量较高。PMF A 和 PMF B 通过口服给药，持续 16 周，用于高脂肪饮食（HFD）诱导肥胖的小鼠。结果表明，PMF B 在 3T3-L1 前体脂肪细胞中比 PMF A 更显著地降低了脂质含量（p < 0.05），减少了脂肪细胞大小，降低了脂肪组织重量，并缓解了 HFD 小鼠的总体体重。PMF A 和 PMF B 通过显著降低脂滴（LD）和 perilipin 1 蛋白和固醇调节元件结合蛋白 1（SREBP-1）表达水平，减少了性腺周围脂肪中的脂肪细胞大小。与 HFD 组相比，PMF B 通过增加普雷沃氏菌和减少 rc4-4 细菌来改变肠道微生物群。肠道微生物群的组成变化、共生和致病微生物的群落可能决定代谢健康，并负责抗肥胖机制。我们的结果表明，柑橘皮提取物可减少体内和体外的脂质积累，应考虑用于超重和肥胖症的管理。
