PRIME, Japan Agency for Medical Research and Development (AMED), Tsukuba, Ibaraki 305-0074, Japan.
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
Int J Syst Evol Microbiol. 2017 May;67(5):1219-1227. doi: 10.1099/ijsem.0.001790. Epub 2017 May 30.
Two bacterial strains, designated EGH7T and TSAH33, were isolated from human faeces and characterized by using a polyphasic taxonomic approach that included analysis of morphology, phenotypic and biochemical features, cellular fatty acid profiles and phylogenetic position based on 16S rRNA and hsp60 gene sequence analyses. The results of 16S rRNA gene sequence analysis indicated that these strains represented members of the family Lachnospiraceae and formed a monophyletic cluster near Eubacterium contortum JCM 6483T (95 % sequence similarity), Ruminococcus gnavus JCM 6515T (95 %), Clostridium oroticum JCM 1429T (95 %), Eubacterium fissicatena JCM 31501T (95 %) and Clostridium nexile JCM 31500T (94 %). The results of a hsp60 gene sequence analysis supported the phylogenetic tree based on the 16S rRNA gene sequence, with a sequence similarity value of between 77.9 and 84.8 % to the five strains listed above. The novel strains were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-stain-positive cocco-bacilli. The strains formed characteristic umbilicated colonies on EG agar plates. The major cellular fatty acids were C18 : 1ω9c, C16 : 0 and C18 : 1ω9c dimethyl acetal (DMA). EGH7T and TSAH33 have DNA G+C contents of 46.9 and 45.5 mol%, respectively. On the basis of these data, strains EGH7T and TSAH33 represent a novel species of a novel genus, for which the name Faecalimonas umbilicata gen. nov., sp. nov. is proposed. The type strain of F. umbilicata is EGH7T (=JCM 30896T=DSM 103426T).
两株细菌菌株，命名为 EGH7T 和 TSAH33，从人类粪便中分离出来，并通过多相分类方法进行了表征，该方法包括形态分析、表型和生化特征、细胞脂肪酸图谱以及基于 16S rRNA 和 hsp60 基因序列分析的系统发育位置。16S rRNA 基因序列分析的结果表明，这些菌株代表 Lachnospiraceae 家族的成员，并在 Eubacterium contortum JCM 6483T（95%序列相似性）、Ruminococcus gnavus JCM 6515T（95%）、Clostridium oroticum JCM 1429T（95%）、Eubacterium fissicatena JCM 31501T（95%）和 Clostridium nexile JCM 31500T（94%）附近形成单系聚类。hsp60 基因序列分析的结果支持基于 16S rRNA 基因序列构建的系统发育树，与上述五个菌株的序列相似性值在 77.9%到 84.8%之间。新型菌株为专性厌氧菌、非色素、非孢子形成、非运动、革兰氏阳性球菌杆菌。菌株在 EG 琼脂平板上形成特征性脐状菌落。主要细胞脂肪酸为 C18:1ω9c、C16:0 和 C18:1ω9c 二甲缩醛（DMA）。EGH7T 和 TSAH33 的 DNA G+C 含量分别为 46.9mol%和 45.5mol%。基于这些数据，菌株 EGH7T 和 TSAH33 代表一个新属的新型种，建议将其命名为 Faecalimonas umbilicata gen. nov.，sp. nov.。F. umbilicata 的模式菌株为 EGH7T（=JCM 30896T=DSM 103426T）。
