基于苯并噻唑衍生物的绿色荧光探针用于活细胞中生物硫醇的成像。
A Green-emitting Fluorescent Probe Based on a Benzothiazole Derivative for Imaging Biothiols in Living Cells.
机构信息
School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou 221116, China.
Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu 476000, China.
出版信息
Molecules. 2019 Jan 23;24(3):411. doi: 10.3390/molecules24030411.
A new green-emitting fluorescent probe was developed for biothiol detection. The sensing mechanism was considered to be biothiol-induced cleavage of the 2,4-dinitrobenzene- sulfonate group in probe and resulting inhibition of the probe's photoinduced electron transfer (PET) process. Probe exhibited favorable properties such as excellent selectivity, highly sensitive (0.12 µM), large Stokes shift (117 nm) and a remarkable turn-on fluorescence signal (148-fold). Furthermore, confocal fluorescence imaging indicated that probe was membrane-permeable and suitable for visualization of biothiols in living A549 cells.
一种新的绿色荧光探针被开发用于生物硫醇检测。传感机制被认为是生物硫醇诱导探针中 2,4-二硝基苯磺酸盐基团的裂解，并抑制探针的光诱导电子转移（PET）过程。探针具有优异的选择性、高灵敏度（0.12µM）、大斯托克斯位移（117nm）和显著的荧光信号开启（148 倍）等优点。此外，共聚焦荧光成像表明，探针具有细胞膜通透性，适用于活 A549 细胞中生物硫醇的可视化。
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