Hubé Florent, Francastel Claire
University Paris Diderot, Sorbonne Paris Cité, 75205 Paris, France.
Epigénétique et Destin Cellulaire, CNRS UMR 7216, 75013 Paris, France.
Noncoding RNA. 2015 Jul 3;1(2):127-138. doi: 10.3390/ncrna1020127.
Currently, the discovery of new small ncRNAs requires high throughput methods even in the case of focused research on the regulation of specific genes or set of genes. We propose herein a simple, rapid, efficient, and cost effective method to clone and sequence single, yet unknown, small ncRNA. This technique that we called "Pocket-sized RNA-Seq" or psRNA-seq is based on transcription, RNA pull down and adapted RACE-PCR methods that allow its implementation using either available commercial kits or in-house reagents.
目前，即使是在专注于特定基因或基因集调控的研究中，新的小非编码RNA的发现也需要高通量方法。在此，我们提出一种简单、快速、高效且经济高效的方法来克隆和测序单个未知的小非编码RNA。我们将这种技术称为“袖珍RNA测序”（psRNA-seq），它基于转录、RNA下拉和改良的RACE-PCR方法，使用现有的商业试剂盒或自制试剂即可实施。
