Agrisani Alberto, Tafer Hakim, Stadler Peter F, Furia Maria
Department of Biology, University of Naples "Federico II", Complesso Universitario Monte Santangelo, via Cinthia, I-80126 Napoli, Italy.
Institut für Biotechnologie, Universität für Bodenkultur, Muthgasse 18, A-1190 Wien, Austria.
Noncoding RNA. 2015 Jul 13;1(2):139-150. doi: 10.3390/ncrna1020139.
A computational screen for novel small nucleolar RNAs in Drosophila melanogaster uncovered 15 novel snoRNAs and snoRNA-like long non-coding RNAs. In contrast to earlier surverys, the novel sequences are mostly poorly conserved and originate from unusual genomic locations. The majority derive from precurors antisense to well-known protein-coding genes, and four of the candidates are produced from exon-coding regions. Only a minority of the new sequences appears to have canonical target sites in ribosomal or small nuclear RNAs. Taken together, these evolutionary young, poorly conserved, and genomically atypical sequences point at a class of snoRNA-like transcripts with predominantly regulatory functions in the fruit fly genome.
一项针对黑腹果蝇新型小核仁RNA的计算筛选发现了15种新型snoRNA和snoRNA样长链非编码RNA。与早期的研究不同，这些新序列大多保守性较差，且起源于不同寻常的基因组位置。大多数序列来自与知名蛋白质编码基因反义的前体，其中四个候选序列由外显子编码区域产生。只有少数新序列似乎在核糖体RNA或小核RNA中有典型的靶位点。综上所述，这些进化上年轻、保守性差且基因组非典型的序列表明，在果蝇基因组中存在一类主要具有调控功能的snoRNA样转录本。
