Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China.
Molecules. 2018 May 26;23(6):1276. doi: 10.3390/molecules23061276.
Franchet, which belongs to the family Liliaceae, is an endangered plant native to China. The wild populations of have been largely reduced by habitat degradation or loss. In our study, we determined the whole chloroplast genome sequence for and compared its structure with other (including ) species. The chloroplast genome of is a circular structure and 152,784 bp in length. The large single copy and small single copy is 82,429 bp and 17,533 bp in size, respectively, and the inverted repeats are 26,411 bp in size. The chloroplast genome contains 116 different genes, including 78 protein coding genes, 30 tRNA genes, 4 rRNA genes, and 4 pseudogenes. There were 51 SSRs detected in the chloroplast genome sequence. Genic comparison among with other (including ) chloroplast genomes shows that the sequence lengths and gene contents show little variation, the only differences being in three pseudogenes. Phylogenetic analysis revealed that was a sister species to . Overall, this study, providing genomic resources and the comparative analysis of chloroplast genomes, will be beneficial for the evolutionary study and phylogenetic reconstruction of the genus , molecular barcoding in population genetics.
Franchet 属于百合科，是中国特有的濒危植物。其野生种群由于栖息地退化或丧失而大量减少。在我们的研究中，我们测定了 Franchet 的完整叶绿体基因组序列，并将其结构与其他 （包括 ）物种进行了比较。Franchet 的叶绿体基因组是一个圆形结构，大小为 152784 bp。大、小单拷贝分别为 82429 bp 和 17533 bp，反向重复序列大小为 26411 bp。Franchet 的叶绿体基因组包含 116 个不同的基因，包括 78 个蛋白编码基因、30 个 tRNA 基因、4 个 rRNA 基因和 4 个假基因。在 Franchet 的叶绿体基因组序列中检测到 51 个 SSRs。Franchet 与其他 （包括 ）叶绿体基因组的基因比较表明，序列长度和基因含量变化很小，仅在三个假基因上存在差异。系统发育分析表明，Franchet 是 （植物名称）的姐妹种。总的来说，这项研究提供了 Franchet 的基因组资源和叶绿体基因组的比较分析，将有助于该属的进化研究和系统发育重建、群体遗传学中的分子标记。
