Departamento de Biologia Geral, Universidade Federal de Minas Gerais, CP 486, Belo Horizonte, MG, 31270-901, Brazil.
Universidade de São Paulo, Instituto de Biociências, Departamento de Botânica, São Paulo, São Paulo, Brazil.
Sci Rep. 2018 Feb 2;8(1):2210. doi: 10.1038/s41598-018-20189-4.
Byrsonima is the third largest genus (about 200 species) in the Malpighiaceae family, and one of the most common in Brazilian savannas. However, there is no molecular phylogeny available for the genus and taxonomic uncertainties at the generic and family level still remain. Herein, we sequenced the complete chloroplast genome of B. coccolobifolia and B. crassifolia, the first ones described for Malpighiaceae, and performed comparative analyses with sequences previously published for other families in the order Malpighiales. The chloroplast genomes assembled had a similar structure, gene content and organization, even when compared with species from other families. Chloroplast genomes ranged between 160,212 bp in B. crassifolia and 160,329 bp in B. coccolobifolia, both containing 115 genes (four ribosomal RNA genes, 28 tRNA genes and 83 protein-coding genes). We also identified sequences with high divergence that might be informative for phylogenetic inferences in the Malpighiales order, Malpighiaceae family and within the genus Byrsonima. The phylogenetic reconstruction of Malpighiales with these regions highlighted their utility for phylogenetic studies. The comparative analyses among species in Malpighiales provided insights into the chloroplast genome evolution in this order, including the presence/absence of three genes (infA, rpl32 and rps16) and two pseudogenes (ycf1 and rps19).
Byrsonima 是卫矛科（Malpighiaceae）中第三大属（约 200 种），也是巴西热带稀树草原最常见的属之一。然而，该属目前还没有分子系统发育，属和科的分类学不确定性仍然存在。本文首次对卫矛科的 B. coccolobifolia 和 B. crassifolia 两个种的完整叶绿体基因组进行了测序，并与已发表的其他目（Malpighiales）科的序列进行了比较分析。组装的叶绿体基因组具有相似的结构、基因含量和组织，即使与其他科的物种相比也是如此。叶绿体基因组在 B. crassifolia 中大小为 160,212bp，在 B. coccolobifolia 中大小为 160,329bp，均包含 115 个基因（4 个核糖体 RNA 基因、28 个 tRNA 基因和 83 个蛋白编码基因）。我们还鉴定出了具有高度差异的序列，这些序列可能对 Malpighiales 目中的系统发育推断具有信息性，对 Malpighiaceae 科和 Byrsonima 属内的系统发育推断也具有信息性。使用这些区域对 Malpighiales 的系统发育重建突出了它们在系统发育研究中的效用。对 Malpighiales 物种间的比较分析提供了对该目中叶绿体基因组进化的深入了解，包括三个基因（infA、rpl32 和 rps16）和两个假基因（ycf1 和 rps19）的存在/缺失。
