Popova P, Vasilyeva L, Tkachuck A, Puzanov M, Golovkin A, Bolotko Y, Pustozerov E, Vasilyeva E, Li O, Zazerskaya I, Dmitrieva R, Kostareva A, Grineva E
Almazov National Medical Research Centre, Saint Petersburg, Russia.
Department of Internal Diseases and Endocrinology, St. Petersburg Pavlov State Medical University, Saint Petersburg, Russia.
Int J Endocrinol. 2018 May 14;2018:6481658. doi: 10.1155/2018/6481658. eCollection 2018.
Our aim was to study the expression of adipokine-encoding genes (leptin, adiponectin, and angiopoietin-like protein 4 (ANGPTL4)) in human umbilical vein endothelial cells (HUVECs) and adipokine concentration in cord blood from women with gestational diabetes mellitus (GDM) depending on glycaemic targets. GDM patients were randomised to 2 groups per target glycaemic levels: GDM1 (tight glycaemic targets, fasting blood glucose < 5.1 mmol/L and <7.0 mmol/L postprandial, = 20) and GDM2 (less tight glycaemic targets, <5.3 mmol/L and < 7.8 mmol/L, respectively, = 21). The control group included 25 women with normal glucose tolerance. ANGPTL4 expression was decreased in the HUVECs from GDM patients versus the control group (23.11 ± 5.71, 21.47 ± 5.64, and 98.33 ± 20.92, for GDM1, GDM2, and controls; < 0.001) with no difference between GDM1 and GDM2. The level of adiponectin gene expression was low and did not differ among the groups. Leptin gene expression was undetectable in HUVECs. In cord blood, leptin/adiponectin ratio (LAR) was increased in GDM2 compared to controls and GDM1 ( = 0.038) and did not differ between GDM1 and controls. Tight glycaemic targets were associated with normalisation of increased LAR in the cord blood. ANGPTL4 expression was downregulated in HUVECs of newborns from GDM mothers and was not affected by the intensity of glycaemic control.
我们的目的是研究脂肪因子编码基因（瘦素、脂联素和血管生成素样蛋白4（ANGPTL4））在人脐静脉内皮细胞（HUVECs）中的表达，以及根据血糖目标，患有妊娠期糖尿病（GDM）的女性脐带血中脂肪因子的浓度。GDM患者根据血糖目标随机分为2组：GDM1组（严格血糖目标，空腹血糖<5.1 mmol/L，餐后<7.0 mmol/L，n = 20）和GDM2组（较宽松血糖目标，分别<5.3 mmol/L和<7.8 mmol/L，n = 21）。对照组包括25名糖耐量正常的女性。与对照组相比，GDM患者的HUVECs中ANGPTL4表达降低（GDM1组、GDM2组和对照组分别为23.11±5.71、21.47±5.64和98.33±20.92；P<0.001），GDM1组和GDM2组之间无差异。脂联素基因表达水平较低，各组之间无差异。在HUVECs中未检测到瘦素基因表达。在脐带血中，与对照组和GDM1组相比，GDM2组的瘦素/脂联素比值（LAR）升高（P = 0.038），GDM1组和对照组之间无差异。严格的血糖目标与脐带血中升高的LAR恢复正常有关。GDM母亲所生新生儿的HUVECs中ANGPTL4表达下调，且不受血糖控制强度的影响。
