Department of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
College of Chemical Engineering, Nanjing Forestry University, No. 159 Longpan Road, Nanjing 210037, China.
Oxid Med Cell Longev. 2018 May 10;2018:3846029. doi: 10.1155/2018/3846029. eCollection 2018.
Oxidative stress and inflammation are considered as two key factors that contribute to the development of atherosclerosis. This study was to investigate the antioxidant capacity of huskless barley and to explore its protective functions through the regulation of the antioxidant defense and inflammatory response in human umbilical vein endothelial cells (HUVEC). The oxygen radical absorbance capacity (ORAC), ferric-reducing antioxidant power (FRAP), and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) scavenging capacity of water and alkali extracts of the polysaccharides from nine huskless barley varieties were investigated . The antioxidant properties of the alkaline extracts were more pronounced than those of the water extracts. The results from the cell model showed that pretreatment of HUVEC with the water or alkaline extracts of the polysaccharides from the huskless barley cultivars QHH and NLGL decreased the levels of reactive oxygen species (ROS), monocyte chemotactic protein 1 (MCP-1), and vascular cell adhesion molecule 1 (VCAM-1) but increased the level of superoxide dismutase (SOD) and maintained cell viability. Huskless barley polysaccharide extracts exhibited the vasodilatory effect of inhibiting angiotensin-converting enzyme (ACE) production. These discoveries revealed the potent protective functions of barley in oxidative damage and a potential role for barley in preventing chronic inflammation in cardiovascular diseases.
氧化应激和炎症被认为是导致动脉粥样硬化发展的两个关键因素。本研究旨在探讨无壳大麦的抗氧化能力，并通过调节人脐静脉内皮细胞（HUVEC）的抗氧化防御和炎症反应来探索其保护功能。研究了 9 种无壳大麦品种多糖的水提物和碱提物的氧自由基吸收能力（ORAC）、铁还原抗氧化能力（FRAP）和 2,2-连氮-双（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）清除能力。碱提物的抗氧化性能比水提物更为显著。细胞模型的结果表明，用 QHH 和 NLGL 无壳大麦品种多糖的水或碱提物预处理 HUVEC 可降低活性氧（ROS）、单核细胞趋化蛋白 1（MCP-1）和血管细胞黏附分子 1（VCAM-1）的水平，但可提高超氧化物歧化酶（SOD）的水平并维持细胞活力。无壳大麦多糖提取物具有抑制血管紧张素转化酶（ACE）生成的血管舒张作用。这些发现揭示了大麦在氧化损伤中的强大保护功能，以及大麦在预防心血管疾病慢性炎症中的潜在作用。
