National Engineering and Technology Research Center for Fruits and Vegetables, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
COFCO Nutrition and Health Research Institute Co., Ltd., Beijing 102209, China.
Nutrients. 2022 Feb 11;14(4):762. doi: 10.3390/nu14040762.
The nutritional functions of highland barley (HB) are superior to those of regular cereals and have attracted increasing attention in recent years. The objective of this study was to investigate whether partly milled highland barley (PHB) can regulate the serum glucose and lipid disorders of mice fed a high-fat diet (HFD) and to further explore their potential gut microbiota modulatory effect. Our results showed that PHB supplementation significantly reduced fasting blood glucose (FBG) and improved oral glucose tolerance. Histological observations confirmed the ability of PHB to alleviate liver and intestine damage. Furthermore, the results of 16S amplicon sequencing revealed that PHB prevented a HFD-induced gut microbiota dysbiosis, enriching some beneficial bacteria, such as , and , and reducing several HFD-dependent taxa (, , e, e, and ). In addition, the increase of and presence has a slightly dose-dependent relationship with the amount of the added PHB. Spearman correlation analysis revealed that and were negatively correlated with the blood glucose level of the oral glucose tolerance test. Overall, our results provide important information about the processing of highland barley to retain its hypoglycemic effect and improve its acceptability and biosafety.
青稞的营养功能优于普通谷物，近年来受到越来越多的关注。本研究旨在探讨部分碾磨青稞（PHB）是否可以调节高脂饮食（HFD）喂养小鼠的血清葡萄糖和脂质紊乱，并进一步探索其潜在的肠道微生物群调节作用。我们的结果表明，PHB 补充剂可显著降低空腹血糖（FBG）并改善口服葡萄糖耐量。组织学观察证实了 PHB 缓解肝和肠损伤的能力。此外，16S 扩增子测序的结果表明，PHB 可预防 HFD 诱导的肠道微生物群失调，富集一些有益细菌，如 、 、 ，并减少几种依赖 HFD 的分类群（ 、 、e、e 和 ）。此外， 和 的增加与添加 PHB 的量呈略微剂量依赖性关系。Spearman 相关分析表明 和 与口服葡萄糖耐量试验的血糖水平呈负相关。总体而言，我们的研究结果为保留青稞的降血糖作用以及提高其可接受性和生物安全性提供了重要信息。
