Yang Mandee, Nam Grace Eun, Salamati Atriya, Baldwin Michael, Deng Mengzhao, Liu Zi-Jun
Dept. Orthodontics, School of Dentistry, University of Washington, Seattle, USA.
Dept. Oral Health Sciences, School of Dentistry, University of Washington, Seattle, USA.
Heliyon. 2018 Mar 28;4(3):e00589. doi: 10.1016/j.heliyon.2018.e00589. eCollection 2018 Mar.
To address how experimental periodontal disease affects alveolar bone mass and mineral apposition in a young pig model.
Seven three-month-old pigs were periodically inoculated with 4 types of periodontal bacteria, along with a ligature around the last maxillary deciduous molar for 8 weeks to induce periodontal disease (PG). Eight same-aged pigs served as the control (CG). Segmentations of 3D cone-beam CT images were performed to quantify volumes of the total alveolar bone, alveolar ridge, and all roots of the target molar. Calcein and alizarin were administered for labeling mineral apposition before euthanasia. The harvested molar blocks were sectioned and examined under epifluorescence. The inter-label distance between the two vital markers at regional bone surfaces were measured and mineral apposition rate (MAR) was calculated.
A significant reduction of total alveolar bone volume was seen in PG with the major loss at the alveolar ridge. MAR was significantly higher at the root furcation region than those at both buccal and palatal ridges in CG. Compared with CG, PG animals showed more interrupted labeled bands with significantly lower MAR at the furcation region. MARs were positively associated with both the volumes of total alveolar bone and ridge in CG, but only with the total alveolar bone in PG.
In young growing pigs, mineral apposition is region specific. The experimental periodontal disease not only leads to alveolar bone loss, but also perturbs mineral apposition for new bone formation, thus impairing the homeostasis of alveolar bone remodeling.
探讨实验性牙周病如何影响幼猪模型中的牙槽骨量和矿物质沉积。
七只三个月大的猪定期接种4种牙周细菌，并在上颌最后一颗乳牙周围结扎8周以诱导牙周病（PG组）。八只同龄猪作为对照组（CG组）。对三维锥形束CT图像进行分割，以量化总牙槽骨、牙槽嵴和目标磨牙所有牙根的体积。在安乐死之前给予钙黄绿素和茜素进行矿物质沉积标记。收获的磨牙块进行切片并在落射荧光下检查。测量区域骨表面两个活性标记之间的标记间距，并计算矿物质沉积率（MAR）。
PG组总牙槽骨体积显著减少，主要在牙槽嵴处丢失。CG组根分叉区域的MAR显著高于颊侧和腭侧牙槽嵴处。与CG组相比，PG组动物在分叉区域的标记带中断更多，MAR显著更低。CG组中MAR与总牙槽骨和牙槽嵴体积均呈正相关，但PG组中仅与总牙槽骨呈正相关。
在幼龄生长猪中矿物质沉积具有区域特异性。实验性牙周病不仅导致牙槽骨丢失，还扰乱新骨形成的矿物质沉积，从而损害牙槽骨重塑的稳态。
