Song Pengyan, Li Danyang, Wang Xiaodan, Zhong Xiuhui
College of Traditional Chinese Veterinary Medicine, Agricultural University of Hebei, Baoding, China.
Andrologia. 2018 Oct;50(8):e13059. doi: 10.1111/and.13059. Epub 2018 Jun 3.
This study was conducted to explore the effects of maternal exposure to perfluorooctanoic acid (PFOA) on reproduction and development of male offspring mice. Pregnant mice were given 1, 2.5 or 5 mg/kg BW PFOA daily by gavage during gestation. The results showed that the survival number of offspring mice at weaning was significantly decreased. There were no differences in the testicular index of offspring mice between PFOA exposure groups and non-PFOA group. Maternal exposure to PFOA reduced the level of testosterone in the male offspring mice on PND 21 (p < 0.01) but increased in 1 mg/kg group and decreased in 2.5 and 5 mg/kg groups on PND 70 (p < 0.01). There were different degrees of damage to testis in a dose-dependent manner, and the number of Leydig cells markedly decreased (p < 0.01) in 2.5 and 5 mg/kg PFOA groups on PND 21 and PND 70. The expression of Dlk1-Dio3 imprinted gene cluster showed a decreasing trend, where Glt2, Rian and Dio3 gene expressions were significantly reduced (p < 0.05) on PND 21. Therefore, PFOA exposure during pregnancy reduces the number of survival offspring mice, damages testis, disrupts reproductive hormones and reduces the mRNA expressions of the Dlk1-Dio3 imprinted cluster in testis.
本研究旨在探讨母体暴露于全氟辛酸（PFOA）对雄性子代小鼠生殖和发育的影响。在妊娠期，通过灌胃法每日给怀孕小鼠分别给予1、2.5或5 mg/kg体重的PFOA。结果显示，子代小鼠断奶时的存活数量显著减少。PFOA暴露组与非PFOA组子代小鼠的睾丸指数无差异。母体暴露于PFOA可使雄性子代小鼠在出生后第21天的睾酮水平降低（p<0.01），但在出生后第70天，1 mg/kg组升高，2.5和5 mg/kg组降低（p<0.01）。睾丸出现不同程度的损伤，呈剂量依赖性，在出生后第21天和第70天，2.5和5 mg/kg PFOA组的睾丸间质细胞数量显著减少（p<0.01）。Dlk1-Dio3印记基因簇的表达呈下降趋势，其中在出生后第21天，Glt2、Rian和Dio3基因的表达显著降低（p<0.05）。因此，孕期暴露于PFOA会减少子代小鼠的存活数量，损伤睾丸，扰乱生殖激素，并降低睾丸中Dlk1-Dio3印记基因簇的mRNA表达。
