College of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding, 071001, China.
College of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding, 071001, China.
Chemosphere. 2021 Jun;272:129585. doi: 10.1016/j.chemosphere.2021.129585. Epub 2021 Jan 6.
This study was conducted to explore the effects of maternal exposure to perfluorooctanoic acid (PFOA) on testicular development of male offspring mice. 20 pregnant Kunming mice were randomly divided into control group and PFOA exposure group with 10 mice of each. In PFOA exposure group, pregnant mice were given 5 mg/kg BW PFOA daily by gavage during gestation. Male offspring mice were killed to separate serum and collect testis at postpartum day 21, then tested the experimental indicators. The results showed that compared with control group, the content of PFOA in the serum of PFOA-exposed mice increased significantly and testosterone content is significantly reduced. Histological observations revealed architectural damages in testis in PFOA exposed groups and the apoptosis was increased. Transcriptome sequencing results showed that the U4/U6 snRNA coding genes snu13 and prp19 complex coding genes HSP73 were up-regulated and the U5 snRNA coding genes Brr2, Prp8 and EJC/TREX coding THOC genes were down-regulated after PFOA exposure Real-time PCR confirmed this result. These results indicate that the exposure of pregnant mice to perfluorooctanoic acid will have a damaging effect on the development of testes in male offspring mice, which may be due to blocked activation of the shear body, changes in structural functions, and inability to perform shear functions.
本研究旨在探讨母鼠暴露于全氟辛酸（PFOA）对雄性仔鼠睾丸发育的影响。将 20 只昆明孕鼠随机分为对照组和 PFOA 暴露组，每组 10 只。在 PFOA 暴露组中，妊娠小鼠在妊娠期间每天通过灌胃给予 5mg/kg BW PFOA。在产后第 21 天处死雄性仔鼠，分离血清并收集睾丸，然后检测实验指标。结果表明，与对照组相比，PFOA 暴露组母鼠血清中 PFOA 含量明显增加，睾酮含量明显降低。组织学观察显示，PFOA 暴露组睾丸结构受损，凋亡增加。转录组测序结果显示，PFOA 暴露后 U4/U6 snRNA 编码基因 snu13 和 prp19 复合物编码基因 HSP73 上调，U5 snRNA 编码基因 Brr2、Prp8 和 EJC/TREX 编码 THOC 基因下调。实时 PCR 验证了这一结果。这些结果表明，妊娠母鼠暴露于全氟辛酸会对雄性仔鼠睾丸发育造成损害，这可能是由于剪切体激活受阻、结构功能改变以及无法进行剪切功能所致。
