Department of Advanced Materials , Hannam University , Daejeon 34054 , Republic of Korea.
Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital, Institute of Health Sciences , Gyeongsang National University , Jinju 52727 , Republic of Korea.
ACS Appl Mater Interfaces. 2018 Jun 27;10(25):21091-21102. doi: 10.1021/acsami.8b02141. Epub 2018 Jun 12.
Sustained release of bioactive molecules from delivery systems is a common strategy for ensuring their prolonged bioactivity and for minimizing safety issues. However, residual toxic reagents, the use of harsh organic solvents, and complex fabrication procedures in conventional delivery systems are considered enormous impediments toward clinical use. Herein, we describe bone morphogenetic protein-2 (BMP-2)-immobilized porous polycaprolactone particles with unique leaf-stacked structures (LSS particles) prepared using clinically feasible materials and procedures. The BMP-2 immobilized in these LSS particles is continuously released up to 36 days to provide an appropriate environment for osteogenic differentiation of human periosteum-derived cells and new bone formation. Thus, the leaf-stacked structures of these LSS particles provide a simple but clinically applicable platform for effectively delivering a variety of bioactive molecules, such as growth factors, hormones, cytokines, peptides, etc.
从给药系统中持续释放生物活性分子是确保其长期生物活性和最小化安全问题的常用策略。然而，在传统给药系统中，残留的有毒试剂、使用苛刻的有机溶剂和复杂的制造工艺被认为是临床应用的巨大障碍。在此，我们描述了使用临床可行的材料和程序制备的具有独特叶堆叠结构（LSS 颗粒）的骨形态发生蛋白-2（BMP-2）固定多孔聚己内酯颗粒。这些 LSS 颗粒中固定的 BMP-2 持续释放长达 36 天，为人骨膜来源细胞的成骨分化和新骨形成提供了适宜的环境。因此，这些 LSS 颗粒的叶堆叠结构为有效递多种生物活性分子（如生长因子、激素、细胞因子、肽等）提供了一个简单但临床适用的平台。
