Department of Nanobiomedical Science , Dankook University , Cheonan 31116 , Republic of Korea.
Department of Oral and Maxillofacial Surgery , Gyeongsang National University School of Medicine and Gyeongsang National University Hospital, Institute of Health Sciences, Gyeongsang National University , Jinju 52828 , Republic of Korea.
ACS Appl Mater Interfaces. 2018 Sep 12;10(36):30115-30124. doi: 10.1021/acsami.8b09558. Epub 2018 Aug 29.
We developed an asymmetrically porous membrane with a leaf-stacked structure (LSS membrane; top with nanosized pores and bulk/bottom with leaf-stacked structure) via immersion-precipitation using polycarprolactone (PCL)/Pluronic F127 mixture solution (in tetraglycol). The bone morphogenetic protein-2 (BMP-2) is immobilized on the pore surfaces of the LSS membrane by immersing the membrane in the BMP-2 solution. The BMP-2 loaded in the LSS membrane is continuously released for 38 days (without additional modifications of the matrix) to improve osteogenic differentiation of cells and new bone formation (carvarial defect rat model). The leaf-stacked structure is recognized to be a physical stimulus for bone regeneration, and the stimulation effect is comparable to that of continuously released BMP-2. Moreover, we observe the combined effect of BMP-2 and the leaf-stacked structure for bone healing. Thus, we suggest that the BMP-2-immobilized LSS membrane may be a candidate as a bioactive guided bone regeneration (GBR) membrane for clinical applications, due to the use of clinically acceptable biomaterials and fabrication procedures as well as effective osteogenic differentiation and bone regeneration.
我们通过使用聚己内酯 (PCL)/Pluronic F127 混合物溶液（在四甘醇中）进行浸沉沉淀，开发出具有叶堆叠结构的不对称多孔膜（LSS 膜；顶部具有纳米级孔，主体/底部具有叶堆叠结构）。通过将膜浸入 BMP-2 溶液中，将骨形态发生蛋白 2（BMP-2）固定在 LSS 膜的孔表面上。负载在 LSS 膜中的 BMP-2 在 38 天内（无需对基质进行额外修改）持续释放，以改善细胞的成骨分化和新骨形成（颅骨缺损大鼠模型）。叶堆叠结构被认为是骨再生的物理刺激物，其刺激效果可与持续释放的 BMP-2 相媲美。此外，我们观察到 BMP-2 和叶堆叠结构对骨愈合的联合作用。因此，由于使用了临床可接受的生物材料和制造工艺以及有效的成骨分化和骨再生，我们建议将 BMP-2 固定的 LSS 膜用作临床应用的生物活性引导骨再生（GBR）膜的候选物。
