Kim Min Ji, Park Jin-Ho, Lee Jae-Hoon, Kim Hyeonjo, Choi Hyeon-Jong, Lee Hee-Chun, Lee Jin Ho, Byun June-Ho, Oh Se Heang
Department of Nanobiomedical Science, Dankook University, Cheonan 31116, Republic of Korea.
Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital, Institute of Health Sciences, Gyeongsang National University, Jinju 52727, Republic of Korea.
ACS Biomater Sci Eng. 2022 Dec 12;8(12):5233-5244. doi: 10.1021/acsbiomaterials.2c00664. Epub 2022 Nov 16.
Even though bony defects can be recovered to their original condition with full functionality, critical-sized bone injuries continue to be a challenge in clinical fields due to deficiencies in the scaffolding matrix and growth factors at the injury region. In this study, we prepared bone morphogenetic protein-2 (BMP-2)-loaded porous particles as a bioactive bone graft for accelerated bone regeneration. The porous particles with unique leaf-stacked morphology (LSS particles) were fabricated by a simple cooling procedure of hot polycaprolactone (PCL) solution. The unique leaf-stacked structure in the LSS particles provided a large surface area and complex release path for the sufficient immobilization of BMP-2 and sustained release of BMP-2 for 26 days. The LSS was also recognized as a topographical cue for cell adhesion and differentiation. In cell culture and animal study using a canine mandible defect model, BMP-2-immobilized LSS particles provided a favorable environment for osteogenic differentiation of stem cells and bone regeneration. study suggests a dual stimulus of bone mineral-like (leaf-stacked) structure (a physical cue) and continuously supplied BMP-2 (a biological cue) to be the cause of this improved healing outcome. Thus, LSS particles containing BMP-2 can be a promising bioactive grafting material for effective new bone formation.
尽管骨缺损能够恢复到原始状态并具备全部功能，但由于损伤区域的支架基质和生长因子存在缺陷，临界尺寸的骨损伤在临床领域仍然是一项挑战。在本研究中，我们制备了负载骨形态发生蛋白-2（BMP-2）的多孔颗粒作为生物活性骨移植材料，以加速骨再生。具有独特叶状堆积形态的多孔颗粒（LSS颗粒）通过热聚己内酯（PCL）溶液的简单冷却程序制备而成。LSS颗粒中独特的叶状堆积结构提供了较大的表面积和复杂的释放路径，以充分固定BMP-2并使BMP-2持续释放26天。LSS还被认为是细胞黏附和分化的拓扑学线索。在细胞培养以及使用犬下颌骨缺损模型的动物研究中，固定有BMP-2的LSS颗粒为干细胞的成骨分化和骨再生提供了有利环境。该研究表明，骨矿物质样（叶状堆积）结构（物理线索）和持续供应的BMP-2（生物线索）的双重刺激是愈合结果改善的原因。因此，含有BMP-2的LSS颗粒有望成为促进有效新骨形成的生物活性移植材料。
