State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology , Huazhong Agricultural University , Wuhan 430070 , China.
CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica , South China Sea Institute of Oceanology, Chinese Academy of Sciences , 164 West Xingang Road , Guangzhou 510301 , P. R. China.
Org Lett. 2018 Jun 15;20(12):3562-3565. doi: 10.1021/acs.orglett.8b01341. Epub 2018 Jun 4.
The biosynthetic pathway of diisonitrile chalkophore SF2768 was identified in Streptomyces thioluteus through heterologous expression recently. Isolation and structure elucidation of the N-substituted formamides that coexisted with the diisonitriles implied that a hydration event was involved. In vitro enzymatic assays of an endogenous isonitrile hydratase suggested a rare sequential-hydration of the diisonitriles. Additionally, the results of Cu-CAS assays indicate that both partial and complete hydration led to the loss of the copper-chelating ability of SF2768.
最近，通过异源表达，人们在硫链丝菌（Streptomyces thioluteus）中确定了二异腈类 chalkophore SF2768 的生物合成途径。与二异腈类共同存在的 N-取代甲酰胺的分离和结构阐明表明涉及水合反应。对内源异腈水解酶的体外酶促测定表明，二异腈类的水合反应罕见地是顺序进行的。此外，Cu-CAS 测定的结果表明，部分和完全水合都会导致 SF2768 失去与铜配位的能力。
