PhD Candidate, Department of Surgery and Periodontology, Ribeirão Preto School of Dentistry, University of São Paulo-USP, São Paulo, Brazil.
Associate Professor, Department of Restorative Dentistry, Piracicaba School of Dentistry, State University of Campinas-UNICAMP, São Paulo, Brazil.
Implant Dent. 2018 Aug;27(4):461-466. doi: 10.1097/ID.0000000000000783.
The association of doxycycline (DOX) and autogenous bone on repair of critical-size defects was evaluated.
Fifty albino rats were divided into 5 groups (n = 10). A 5-mm diameter defect was treated with: control (CO)-blood clot; DOX in Natrosol (NAT)-10% gel; NAT-gel; particulate autogenous bone (PAB); and PAB + DOX - PAB associated with 10% DOX gel. The animals were euthanized at 4 and 8 weeks postoperatively. Histomorphometric analysis was performed to assess the percentage of new bone in the defect area. Statistical analysis of the results was performed using analysis of variance and the Tukey test (P < 0.05).
The results showed that new bone formation was limited to the margins of the defect. At 4 and 8 weeks, the group PAB + DOX showed higher bone formation (38.59% and 47.86%, respectively), with statistical difference in comparison with the CO (19.52%) at 4 weeks and CO (18.80%), DOX (22.05%), and NAT (15.89%) at 8 weeks (P < 0.05).
The association of 10% DOX with autogenous bone significantly improved bone healing in critical-size defects.
评估盐酸多西环素（DOX）与自体骨在修复临界尺寸缺损中的联合作用。
将 50 只白化大鼠分为 5 组（n = 10）。采用以下方法处理 5mm 直径的缺损：对照组（CO）-血凝块；Natrosol（NAT）-10%凝胶中的 DOX；NAT 凝胶；颗粒状自体骨（PAB）；以及与 10% DOX 凝胶相关的 PAB+DOX-PAB。术后 4 周和 8 周时处死动物。进行组织形态计量学分析以评估缺损区域内新骨的百分比。使用方差分析和 Tukey 检验对结果进行统计分析（P < 0.05）。
结果表明，新骨形成仅限于缺损的边缘。在 4 周和 8 周时，PAB+DOX 组的骨形成率更高（分别为 38.59%和 47.86%），与 CO 组（4 周时为 19.52%）以及 CO 组（18.80%）、DOX 组（22.05%）和 NAT 组（15.89%）在 8 周时均有统计学差异（P < 0.05）。
将 10% DOX 与自体骨联合使用可显著改善临界尺寸缺损中的骨愈合。
