Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
Microb Pathog. 2018 Aug;121:318-324. doi: 10.1016/j.micpath.2018.06.004. Epub 2018 Jun 2.
The current study was to identify a protective role of Shuanghuanglian (SHL) injection powder in vitro and in vivo after H5N1 viral infection. Immunofluorescent staining was used to determine the susceptibility of rat intestinal mucosa microvascular endothelial cells (RIM-MVECs) to the H5N1 virus. Viral replication of RIM-MVECs was measured by transmission electron microscopy (TEM) a hemagglutination assay and real-time quantitative PCR. H5N1 virally infected RIM-MVECs, and BALB/c mice were treated with SHL to investigate its therapeutic effect. Animal survival and the weight of H5N1 virally infected BALB/c mice after SHL treatment was noted, and histology and real-time PCR applied to mouse lungs were used to confirm the anti-H5N1 viral effects of SHL. RIM-MVECs supported replication of the H5N1 virus in vitro. SHL treatment reduced viral titers in H5N1 virally infected RIM-MVECs and mouse lungs. SHL -treated mice survived compared to controls. Mild pathological changes, reduced inflammatory cell infiltration and fewer viral antigens were observed in the lungs of SHL-treated mice at days 3 and 6 post-infection. In conclusion, SHL may have the antiviral activity against the H5N1 virus infection by inhibiting viral replication and alleviating lung injury.
本研究旨在鉴定双黄连（SHL）注射液在 H5N1 病毒感染后的体外和体内的保护作用。免疫荧光染色用于确定大鼠肠黏膜微血管内皮细胞（RIM-MVECs）对 H5N1 病毒的易感性。通过透射电子显微镜（TEM）、血凝试验和实时定量 PCR 测量 RIM-MVECs 的病毒复制。用 SHL 处理 H5N1 病毒感染的 RIM-MVECs 和 BALB/c 小鼠，以研究其治疗效果。观察 SHL 处理后 H5N1 病毒感染的 BALB/c 小鼠的动物存活率和体重，并对小鼠肺部进行组织学和实时 PCR 分析，以确认 SHL 对 H5N1 病毒的抗病毒作用。RIM-MVECs 支持 H5N1 病毒在体外的复制。SHL 处理降低了 H5N1 病毒感染的 RIM-MVECs 和小鼠肺部的病毒滴度。与对照组相比，接受 SHL 治疗的小鼠存活。在感染后第 3 天和第 6 天，接受 SHL 治疗的小鼠肺部观察到轻度的病理变化、炎症细胞浸润减少和病毒抗原减少。总之，SHL 可能通过抑制病毒复制和减轻肺损伤对 H5N1 病毒感染具有抗病毒活性。
