细胞色素c氧化酶质子泵的新见解。
New insights on the cytochrome c oxidase proton pump.
作者信息
Thelen M, O'Shea P S, Azzi A
出版信息
Biochem J. 1985 Apr 1;227(1):163-7. doi: 10.1042/bj2270163.
Abstract
Cytochrome c oxidase vesicles were used to show that, under appropriate experimental conditions: (1) no net deprotonation of the vesicular membrane or of the incorporated enzyme occurs during the oxidation of ferrocytochrome c; (2) the pH equilibration kinetics of a respiration-induced pH gradient across the bilayer are a simple function of the ohmic proton-conductance properties of the membrane; (3) a fairly constant stoichiometry (0.8-0.7) of the numbers of protons pumped per molecule of ferrocytochrome c oxidized, i.e. the H+/e- ratio, over a wide range of dioxygen molecules reduced (1-12) is observed.
摘要
细胞色素c氧化酶囊泡被用于证明，在适当的实验条件下：(1) 在亚铁细胞色素c氧化过程中，囊泡膜或掺入的酶没有净去质子化现象发生；(2) 呼吸诱导的跨双层pH梯度的pH平衡动力学是膜的欧姆质子传导特性的简单函数；(3) 在广泛的被还原的双氧分子数量范围（1 - 12）内，观察到每氧化一分子亚铁细胞色素c所泵出的质子数量具有相当恒定的化学计量比（0.8 - 0.7），即H⁺/e⁻ 比。
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