Department of Animal Physiology, Veterinary Medicine School, Complutense University of Madrid, Madrid, Spain.
Department of Animal Medicine, Surgery and Pathology, Veterinary Medicine School, Complutense University of Madrid, Madrid, Spain.
BMC Cancer. 2018 Jun 4;18(1):626. doi: 10.1186/s12885-018-4518-z.
Indole-3-carbinol, derived from Cruciferous vegetables is an estrogen receptor antagonist considered a preventive agent that is naturally present in diet. There are no previous studies on its effects in human inflammatory breast cancer or canine inflammatory mammary cancer that is the most aggressive type of breast cancer.
The aim of this study was to analyze the effect of indole-3-carbinol on a SCID mice xenograft model of canine inflammatory mammary cancer, using equivalent human oral dose as a preventive therapy in humans for 3 weeks.
Indole-3-carbinol treatment decreased tumor proliferation and increased apoptosis, although tumor embolization and liver metastasis were observed in some animals. There was a characteristic subpopulation of lipid-rich cells and increased contents of select steroid hormones in tumor homogenates and serum.
Our data reveal for the first time that the ingestion of indole-3-carbinol, as administered, diminishes proliferation and increases apoptosis of tumor cells in an experimental model of inflammatory breast cancer, although this effect could not be enough to avoid the appearance of tumor embolization and metastasis. Future clinical trials will be needed to clarify the usefulness of indole-3-carbinol in this cancer and to understand the molecular mechanisms involved.
吲哚-3-甲醇，来源于十字花科蔬菜，是一种雌激素受体拮抗剂，被认为是一种天然存在于饮食中的预防剂。以前没有关于其在人类炎性乳腺癌或犬炎性乳腺癌（最具侵袭性的乳腺癌类型）中的作用的研究。
本研究旨在分析吲哚-3-甲醇对犬炎性乳腺癌 SCID 小鼠异种移植模型的影响，使用相当于人类口服剂量的预防性治疗 3 周。
吲哚-3-甲醇治疗降低了肿瘤增殖并增加了细胞凋亡，但在一些动物中观察到肿瘤栓塞和肝转移。肿瘤匀浆和血清中存在特征性的富含脂质的细胞亚群和选定类固醇激素含量增加。
我们的数据首次揭示，在炎性乳腺癌的实验模型中，摄入吲哚-3-甲醇可减少肿瘤细胞的增殖并增加细胞凋亡，尽管这种作用可能不足以避免肿瘤栓塞和转移的出现。未来的临床试验将需要阐明吲哚-3-甲醇在这种癌症中的有用性，并了解涉及的分子机制。
