Cao Binrui, Xu Hong, Yang Mingying, Mao Chuanbin
Department of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, Institute for Biomedical Engineering, Science and Technology, University of Oklahoma, Norman, OK, USA.
Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Hangzhou, China.
Methods Mol Biol. 2018;1776:643-652. doi: 10.1007/978-1-4939-7808-3_41.
Cancer photodynamic therapy (PDT) involves the absorption of light by photosensitizers (PSs) to generate cytotoxic singlet oxygen for killing cancer cells. The success of this method is usually limited by the lack of selective accumulation of the PS at cancer cells. Bioengineered viruses with cancer cell-targeting peptides fused on their surfaces are great drug carriers that can guide the PS to cancer cells for targeted cancer treatment. Here, we use cell-targeting fd bacteriophages (phages) as an example to describe how to chemically conjugate PSs (e.g., pyropheophorbide-a (PPa)) onto a phage particle to achieve targeted PDT.
癌症光动力疗法（PDT）涉及光敏剂（PSs）吸收光以产生活性氧来杀死癌细胞。该方法的成功通常受到PS在癌细胞中缺乏选择性积累的限制。表面融合有癌细胞靶向肽的生物工程病毒是很好的药物载体，可将PS引导至癌细胞用于靶向癌症治疗。在此，我们以靶向细胞的fd噬菌体为例，描述如何将PS（如焦脱镁叶绿酸-a（PPa））化学偶联到噬菌体颗粒上以实现靶向PDT。
