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磷霉素抗性转座子Tn2921的结构与功能分析

Structural and functional analyses of the fosfomycin resistance transposon Tn2921.

作者信息

Navas J, García-Lobo J M, León J, Ortíz J M

出版信息

J Bacteriol. 1985 Jun;162(3):1061-7. doi: 10.1128/jb.162.3.1061-1067.1985.

DOI:10.1128/jb.162.3.1061-1067.1985
PMID:2987181
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215883/
Abstract

The fosfomycin resistance transposon Tn2921 is flanked by directly repeated sequences homologous to the Tn10-related insertion sequence IS10. The nonrepeated DNA sequences of Tn2921 can be deleted without affecting the transposition ability of the element, showing that at least one of the direct repeats is an active insertion sequence. Transposition of Tn2921 seems to occur through direct transposition, since cointegrates have not been observed. The evolutionary relatedness of Tn2921 and IS10 is discussed.

摘要

磷霉素抗性转座子Tn2921两侧是与Tn10相关的插入序列IS10同源的正向重复序列。Tn2921的非重复DNA序列可以被删除而不影响该元件的转座能力,这表明至少有一个正向重复序列是一个活性插入序列。由于未观察到共整合体,Tn2921的转座似乎通过直接转座发生。文中讨论了Tn2921与IS10的进化相关性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/310f/215883/bc10d614298a/jbacter00223-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/310f/215883/8796c958695d/jbacter00223-0216-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/310f/215883/6ec4da93a5cb/jbacter00223-0217-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/310f/215883/bc10d614298a/jbacter00223-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/310f/215883/8796c958695d/jbacter00223-0216-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/310f/215883/6ec4da93a5cb/jbacter00223-0217-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/310f/215883/bc10d614298a/jbacter00223-0218-a.jpg

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本文引用的文献

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Plasmid-determined resistance to fosfomycin in Serratia marcescens.粘质沙雷氏菌中质粒介导的对磷霉素的耐药性。
Antimicrob Agents Chemother. 1980 Aug;18(2):215-9. doi: 10.1128/AAC.18.2.215.
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cea-kil operon of the ColE1 plasmid.ColE1质粒的cea-kil操纵子
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Nucleotide sequence and intracellular location of the product of the fosfomycin resistance gene from transposon Tn2921.转座子Tn2921中磷霉素抗性基因产物的核苷酸序列及细胞内定位
Antimicrob Agents Chemother. 1990 Oct;34(10):2016-8. doi: 10.1128/AAC.34.10.2016.
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Plasmid-encoded fosfomycin resistance.质粒编码的磷霉素耐药性
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Plasmid. 1982 Nov;8(3):276-86. doi: 10.1016/0147-619x(82)90065-8.
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DNA sequence organization of IS10-right of Tn10 and comparison with IS10-left.Tn10右侧IS10的DNA序列组织及其与IS10左侧的比较。
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2608-12. doi: 10.1073/pnas.79.8.2608.
6
Tn292l, a transposon encoding fosfomycin resistance.Tn292l,一种编码磷霉素抗性的转座子。
J Bacteriol. 1982 Jul;151(1):477-9. doi: 10.1128/jb.151.1.477-479.1982.
7
Transposable elements in prokaryotes.原核生物中的转座元件。
Annu Rev Genet. 1981;15:341-404. doi: 10.1146/annurev.ge.15.120181.002013.
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Three Tn10-associated excision events: relationship to transposition and role of direct and inverted repeats.三个与Tn10相关的切除事件:与转座的关系以及正向和反向重复序列的作用。
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Mol Gen Genet. 1980;180(1):123-7. doi: 10.1007/BF00267360.