转座子Tn2921中磷霉素抗性基因产物的核苷酸序列及细胞内定位
Nucleotide sequence and intracellular location of the product of the fosfomycin resistance gene from transposon Tn2921.
作者信息
Navas J, León J, Arroyo M, García Lobo J M
机构信息
Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Santander, Spain.
出版信息
Antimicrob Agents Chemother. 1990 Oct;34(10):2016-8. doi: 10.1128/AAC.34.10.2016.
Abstract
An 863-base-pair DNA fragment containing the fosfomycin resistance gene from transposon Tn2921 was sequenced. Analysis of the sequence revealed the presence of a single open reading frame that encoded the FOS polypeptide of 16 kilodaltons from Tn2921. Minicell fractionation studies showed that the FOS protein was located in the bacterial cytoplasm.
摘要
对含有来自转座子Tn2921的磷霉素抗性基因的一段863个碱基对的DNA片段进行了测序。序列分析揭示存在一个单一的开放阅读框，其编码来自Tn2921的16千道尔顿的FOS多肽。小细胞分级分离研究表明，FOS蛋白位于细菌细胞质中。
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