肠杆菌科成员的ilvGEDA操纵子的限制性内切酶分析。
Restriction endonuclease analysis of the ilvGEDA operon of members of the family Enterobacteriaceae.
作者信息
Driver R P, Lawther R P
出版信息
J Bacteriol. 1985 Jun;162(3):1317-9. doi: 10.1128/jb.162.3.1317-1319.1985.
Abstract
Four of the genes required for the biosynthesis of isoleucine and valine form the ilvGEDA operon in Escherichia coli K-12 and Salmonella typhimurium. The structural relationship of these genes was examined in eight other members of the family Enterobacteriaceae by genomic Southern blot hybridization. These genes are contiguous in all the strains examined, and specific restriction sites appear to be highly conserved, indicating the possible functional importance of the DNA sequences of which they are part.
摘要
异亮氨酸和缬氨酸生物合成所需的四个基因在大肠杆菌K-12和鼠伤寒沙门氏菌中形成ilvGEDA操纵子。通过基因组Southern印迹杂交在肠杆菌科的其他八个成员中检查了这些基因的结构关系。在所检查的所有菌株中，这些基因都是相邻的，并且特定的限制性酶切位点似乎高度保守，这表明它们所属的DNA序列可能具有功能重要性。
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