Taillon M P, Gotto D A, Lawther R P
Nucleic Acids Res. 1981 Jul 24;9(14):3419-32. doi: 10.1093/nar/9.14.3419.
The isolation of a lambda gt . ilvGEDA . S.t. hybrid transducing phage has permitted the characterization of the promoter-attenuator region of the ilvGEDA operon of Salmonella typhimurium. In vitro transcription and Southern hybridization indicate that the promoter-attenuator resides on a 400 nucleotide Rsa I restriction fragment. DNA sequence analysis shows only seven base pair differences exist between the DNA sequence of the ilvGEDA promoter-attenuator of S. typhimurium and that previously published for Escherichia coli K12.
λgt.ilvGEDA.S.t. 杂交转导噬菌体的分离，使得对鼠伤寒沙门氏菌ilvGEDA操纵子的启动子 - 弱化子区域得以进行表征。体外转录和Southern杂交表明，启动子 - 弱化子位于一个400个核苷酸的Rsa I限制性片段上。DNA序列分析显示，鼠伤寒沙门氏菌ilvGEDA启动子 - 弱化子的DNA序列与先前发表的大肠杆菌K12的序列之间仅存在七个碱基对的差异。
