Patel D K, Kumar R, Prasad S K, Hemalatha S
Pharmacognosy Research Laboratory, Department of Pharmaceutics, Institute of Technology, Banaras Hindu University, Varanasi-221005, Canada.
Asian Pac J Trop Biomed. 2011 Oct;1(5):395-400. doi: 10.1016/S2221-1691(11)60087-7.
To examine the antioxidant activity and total phenolic content of different solvent fractions of Pedalium murex (P. murex) Linn fruits (Family: Pedaliaceae) as well as the correlation between the total antioxidant capacity and total phenolic content.
In the present study, the antioxidant activities of P. murex were evaluated using six in-vitro assays, namely total antioxidant assay, DPPH assay, reducing power, nitric oxide scavenging, hydrogen peroxide scavenging and deoxyribose scavenging assays, and total phenol contents were also investigated.
The ethyl acetate (EA) fraction was found to have high levels of phenolic content (298.72±2.09 mg GAE/g). The EA fraction exhibit higher total antioxidant capacity, higher percentage of DPPH radical scavenging activity (135.11±2.95µg/mL), nitric oxide (200.57±4.51µg/mL), hydrogen peroxide (217.91±6.12 µg/mL), deoxyribose (250.01±4.68µg/mL) and higher reducing power. Correlation coefficient (r(2) =0.914) was found to be significant between total phenolic content and total antioxidant activity.
In general, the results indicate that the EA fractions are rich in phenolic antioxidants with potent free radical scavenging activity implying their importance to human health.
研究刺蒴麻（Pedalium murex） Linn果实（刺蒴麻科）不同溶剂萃取物的抗氧化活性和总酚含量，以及总抗氧化能力与总酚含量之间的相关性。
在本研究中，使用六种体外试验评估刺蒴麻的抗氧化活性，即总抗氧化试验、DPPH试验、还原能力、一氧化氮清除试验、过氧化氢清除试验和脱氧核糖清除试验，并研究总酚含量。
发现乙酸乙酯（EA）萃取物具有高水平的酚含量（298.72±2.09毫克没食子酸当量/克）。EA萃取物表现出更高的总抗氧化能力、更高的DPPH自由基清除活性百分比（135.11±2.95微克/毫升）、一氧化氮（200.57±4.51微克/毫升）、过氧化氢（217.91±6.12微克/毫升）、脱氧核糖（250.01±4.68微克/毫升）以及更高的还原能力。总酚含量与总抗氧化活性之间的相关系数（r(2)=0.914）被发现具有显著性。
总体而言，结果表明EA萃取物富含具有强大自由基清除活性的酚类抗氧化剂，这意味着它们对人类健康具有重要意义。
