Graham J S, Pearce G, Merryweather J, Titani K, Ericsson L, Ryan C A
J Biol Chem. 1985 Jun 10;260(11):6555-60.
A cDNA containing the coding region for the complete amino acid sequence of wound-induced proteinase Inhibitor I from tomato leaves was constructed in the plasmid pUC9 and characterized. The open reading frame codes for a protein of 111 amino acids. This deduced amino acid sequence revealed the presence of a 42-amino acid N-terminal sequence that is not found in the native protein. This sequence appears to contain a 23-amino acid segment typical of a signal sequence followed by a 19-amino acid sequence containing 9 charged amino acids. The 42-amino acid sequence is apparently lost during maturation to the native Inhibitor I and represents 38% of the translated protein. The Inhibitor I amino acid sequence contains 71% identity with potato tuber Inhibitor I sequence and 35% identity with an inhibitor from the leech.
一个包含番茄叶片伤口诱导蛋白酶抑制剂I完整氨基酸序列编码区的cDNA被构建到质粒pUC9中并进行了表征。开放阅读框编码一个111个氨基酸的蛋白质。这个推导的氨基酸序列显示存在一个42个氨基酸的N端序列，该序列在天然蛋白质中不存在。该序列似乎包含一个典型的23个氨基酸的信号序列片段，后面跟着一个包含9个带电荷氨基酸的19个氨基酸序列。这个42个氨基酸的序列在成熟为天然抑制剂I的过程中显然丢失了，占翻译后蛋白质的38%。抑制剂I的氨基酸序列与马铃薯块茎抑制剂I序列有71%的同一性，与水蛭的一种抑制剂有35%的同一性。
