Keil M, Sanchez-Serrano J, Schell J, Willmitzer L
Nucleic Acids Res. 1986 Jul 25;14(14):5641-50. doi: 10.1093/nar/14.14.5641.
The isolation and characterization of a genomic clone encoding proteinase inhibitor II of potato (Solanum tuberosum) is described. The structure of this gene was determined by sequencing a genomic fragment of about 2 kb containing the entire RNA coding as well as about 900 nucleotides of the 5'-upstream and 250 nucleotides of the 3'-downstream region. The transcription start site was determined by RNase protection experiments. The comparison of the genomic sequence with cDNA sequences reveals the presence of one intron with a length of 117 nucleotides. The genomic clone contains an open reading frame of 462 nucleotides allowing for a protein of 154 amino acids. The proteinase inhibitor II gene displays typical features of eucaryotic genes. The sequence TATAAA is found 26 nucleotides upstream of the transcription initiation site and the sequence CAAAT at position--103. In the 3'-region the sequence AATAA is found 33 nucleotides in front of the poly-A addition site.
本文描述了马铃薯（茄属）蛋白酶抑制剂II基因组克隆的分离与鉴定。通过对一个约2 kb的基因组片段进行测序来确定该基因的结构，该片段包含整个RNA编码区以及约900个核苷酸的5'上游区域和250个核苷酸的3'下游区域。转录起始位点通过核糖核酸酶保护实验确定。基因组序列与cDNA序列的比较揭示了存在一个长度为117个核苷酸的内含子。该基因组克隆包含一个462个核苷酸的开放阅读框，编码一个由154个氨基酸组成的蛋白质。蛋白酶抑制剂II基因表现出真核基因的典型特征。在转录起始位点上游26个核苷酸处发现序列TATAAA，在-103位置处发现序列CAAAT。在3'区域，在多聚腺苷酸添加位点前33个核苷酸处发现序列AATAA。
