S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-letya Octyabrya 7/2, Moscow 117312, Russia.
Laboratory of Environmental Microbiology, Institute of Microbiology of the ASCR, Vídeňská 1083, 14220 Prague 4, Czech Republic.
Int J Syst Evol Microbiol. 2012 Jul;62(Pt 7):1512-1520. doi: 10.1099/ijs.0.034819-0. Epub 2011 Aug 19.
Two strains of subdivision 1 Acidobacteria, a pink-pigmented bacterium KA1(T) and a colourless isolate WH120(T), were obtained from acidic Sphagnum peat and wood under decay by the white-rot fungus Hyploma fasciculare, respectively. Cells of these isolates were Gram-negative-staining, non-motile, short rods, which were covered by large polysaccharide capsules and occurred singly, in pairs, or in short chains. Strains KA1(T) and WH120(T) were strictly aerobic mesophiles that grew between 10 and 33 °C, with an optimum at 22-28 °C. Both isolates developed under acidic conditions, but strain WH120(T) was more acidophilic (pH growth range 3.5-6.4; optimum, 4.0-4.5) than strain KA1(T) (pH growth range 3.5-7.3; optimum , 5.0-5.5). The preferred growth substrates were sugars. In addition, the wood-derived isolate WH120(T) grew on oxalate, lactate and xylan, while the peat-inhabiting acidobacterium strain KA1(T) utilized galacturonate, glucuronate and pectin. The major fatty acids were iso-C(15:0) and iso-C(17:1)ω8c; the cells also contained significant amounts of 13,16-dimethyl octacosanedioic acid. The quinone was MK-8. The DNA G+C contents of strains KA1(T) and WH120(T) were 54.1 and 51.7 mol%, respectively. Strains KA1(T) and WH120(T) displayed 97.8% 16S rRNA gene sequence similarity to each other. The closest recognized relatives were Acidobacterium capsulatum and Telmatobacter bradus (93.4-94.3% 16S rRNA gene sequence similarity). These species differed from strains KA1(T) and WH120(T) by their ability to grow under anoxic conditions, the absence of capsules, presence of cell motility and differing fatty acid composition. Based on these differences, the two new isolates are proposed as representing a novel genus, Acidicapsa gen. nov., and two novel species. Acidicapsa borealis gen. nov., sp. nov. is the type species for the new genus with strain KA1(T) (=DSM 23886(T)=LMG 25897(T)=VKM B-2678(T)) as the type strain. The name Acidicapsa ligni sp. nov. is proposed for strain WH120(T) (=LMG 26244(T)=VKM B-2677(T)=NCCB 100371(T)).
两株酸杆菌亚群 1 的分支，一株是粉红色着色细菌 KA1(T)，另一株是无色分离株 WH120(T)，分别从酸性泥炭和白腐真菌 Hypoloma fasciculare 下腐烂的木材中获得。这些分离株的细胞革兰氏阴性染色，不运动，为短杆状，被大的多糖胶囊覆盖，单独存在、成对存在或成短链存在。菌株 KA1(T)和 WH120(T)均为严格需氧的中温微生物，在 10 至 33°C 之间生长，最适温度为 22-28°C。两种分离株都在酸性条件下生长，但 WH120(T)比 KA1(T)更嗜酸（pH 生长范围 3.5-6.4；最佳 pH 值为 4.0-4.5）。菌株 WH120(T)（pH 生长范围 3.5-7.3；最佳 pH 值为 5.0-5.5）比菌株 KA1(T)（pH 生长范围 3.5-7.3）更嗜酸。优选的生长底物是糖。此外，来源于木材的分离株 WH120(T)在草酸盐、乳酸盐和木聚糖上生长，而栖息在泥炭中的酸杆菌菌株 KA1(T)利用半乳糖醛酸盐、葡萄糖醛酸盐和果胶。主要脂肪酸为 iso-C(15:0)和 iso-C(17:1)ω8c；细胞还含有大量的 13,16-二甲基辛二酸。醌为 MK-8。菌株 KA1(T)和 WH120(T)的 DNA G+C 含量分别为 54.1mol%和 51.7mol%。菌株 KA1(T)和 WH120(T)的 16S rRNA 基因序列相似度为 97.8%。最接近的公认亲缘种是 Acidobacterium capsulatum 和 Telmatobacter bradus（16S rRNA 基因序列相似度为 93.4-94.3%）。这些种与菌株 KA1(T)和 WH120(T)的区别在于它们在缺氧条件下的生长能力、胶囊的不存在、细胞运动性的存在以及不同的脂肪酸组成。基于这些差异，这两个新的分离株被提议代表一个新属，酸性囊菌属，和两个新种。北方酸性囊菌属，新属，sp. nov. 是新属的模式种，以 KA1(T)（=DSM 23886(T)=LMG 25897(T)=VKM B-2678(T)）作为模式株。WH120(T)（=LMG 26244(T)=VKM B-2677(T)=NCCB 100371(T)）被提议为木质酸性菌新种。
