J Agric Food Chem. 2018 Jun 27;66(25):6336-6347. doi: 10.1021/acs.jafc.8b02502. Epub 2018 Jun 15.
Cutleaf groundcherry ( Physalis angulata L.) is an annual plant with a number of medicinal ingredients. However, studies about the secondary metabolism of P. angulata are very limited. An integrated metabolome and proteome approach was used to reveal the variations in the metabolism associated with bioactive compounds under methyl-jasmonate (MeJA) treatment. Application of MeJA to the hairy roots could significantly increase the accumulation of most active ingredients. A targeted approach confirmed the variations in physalins D and H between MeJA treatment and the controls. Increases in the levels of a number of terpenoid backbone biosynthesis and steroid biosynthesis related enzymes, cytochrome P450 monooxygenases and 3β-hydroxysterioid dehydrogenase might provide a potential explanation for the MeJA-induced active ingredient synthesis. Our results may contribute to a deeper understanding of the regulation mechanism underlying the MeJA-induced active compound accumulation in P. angulata.
去叶酸浆（Physalis angulata L.）是一种一年生植物，含有多种药用成分。然而，关于 P. angulata 次生代谢的研究非常有限。本研究采用整合代谢组学和蛋白质组学方法，揭示了甲基茉莉酸（MeJA）处理下与生物活性化合物相关的代谢变化。MeJA 处理显著增加了发根中大多数活性成分的积累。靶向分析证实了 MeJA 处理与对照之间 physalins D 和 H 的变化。一些萜类化合物骨架生物合成和甾体生物合成相关酶、细胞色素 P450 单加氧酶和 3β-羟甾醇脱氢酶水平的增加可能为 MeJA 诱导活性成分合成提供了潜在解释。我们的结果可能有助于深入了解 MeJA 诱导 P. angulata 中活性化合物积累的调控机制。
