Zhang Lihua, Yun Yueli, Hu Guowen, Peng Yu
Centre for Behavioral Ecology and Evolution College of Life Sciences Hubei University Wuhan China.
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources College of Life Sciences Hubei University Wuhan China.
Ecol Evol. 2018 Apr 19;8(10):4899-4906. doi: 10.1002/ece3.4051. eCollection 2018 May.
Most spiders are natural enemies of pests, and it is beneficial for the biological control of pests to learn the relationships between symbionts and their spider hosts. Research on the bacterial communities of insects has been conducted recently, but only a few studies have addressed the bacterial communities of spiders. To obtain a complete overview of the microbial communities of spiders, we examined eight species of spider (, , , , , , and ) with high-throughput sequencing based on the V3 and V4 regions of the 16S rRNA gene. The bacterial communities of the spider samples were dominated by five types of endosymbionts, , , , and . The dominant OTUs (operational taxonomic units) from each of the five endosymbionts were analyzed, and the results showed that different spider species were usually dominated by special OTUs. In addition to endosymbionts, , , , , , , , , , , , , , and were detected in spider samples in our study. Moreover, the abundance of , , and in the spider was significantly higher (<.05) than the bacterial abundance of these species in seven other spider species. These findings suggest that same as in insects, co-infection of multiple types of endosymbionts is common in the hosts of the Araneae order, and other bacterial taxa also exist in spiders besides the endosymbionts.
大多数蜘蛛是害虫的天敌，了解共生体与其蜘蛛宿主之间的关系有助于害虫的生物防治。最近已经对昆虫的细菌群落进行了研究，但只有少数研究涉及蜘蛛的细菌群落。为了全面了解蜘蛛的微生物群落，我们基于16S rRNA基因的V3和V4区域，通过高通量测序对8种蜘蛛（，，，，，，和）进行了检测。蜘蛛样本的细菌群落主要由5种内共生体，，，和主导。对这5种内共生体各自的优势OTU（操作分类单元）进行了分析，结果表明不同的蜘蛛物种通常由特定的OTU主导。除了内共生体，在我们的研究中，蜘蛛样本还检测到了，，，，，，，，，，，，，和。此外，蜘蛛中，，和的丰度显著高于其他7种蜘蛛中这些物种的细菌丰度（<.05）。这些发现表明，与昆虫一样，多种类型内共生体的共同感染在蜘蛛目宿主中很常见，并且除了内共生体之外，蜘蛛中还存在其他细菌类群。
