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嗜鸟粪盘菌核糖体DNA重复单元的克隆与特性分析

Cloning and characterization of the rDNA repeat unit of Podospora anserina.

作者信息

Labat N, Perrot M, Bégueret J

出版信息

Mol Gen Genet. 1985;199(1):154-7. doi: 10.1007/BF00327526.

DOI:10.1007/BF00327526
PMID:2987647
Abstract

DNA coding for ribosomal RNA in Podospora anserina has been cloned and was found as a tandemly repeated 8.3 kb sequence. The cloned rDNA was characterized by restriction endonuclease mapping. The location of 5.8S, 18S and 28S rRNA coding regions was established by DNA-RNA hybridization and S1 nuclease mapping. The organization of P. anserina rRNA genes is similar to that of Neurospora crassa and Aspergillus nidulans. The rDNA unit does not contain the sequence coding for 5S RNA.

摘要

已克隆了嗜鸟粪盘菌中编码核糖体RNA的DNA,发现其为一个串联重复的8.3 kb序列。通过限制性内切酶图谱分析对克隆的核糖体DNA进行了表征。通过DNA-RNA杂交和S1核酸酶图谱分析确定了5.8S、18S和28S核糖体RNA编码区的位置。嗜鸟粪盘菌核糖体RNA基因的组织方式与粗糙脉孢菌和构巢曲霉相似。核糖体DNA单元不包含编码5S RNA的序列。

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本文引用的文献

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Altered ribosomal proteins in emetine resistant strains in the fungus Podospora anserina.蛹草拟青霉中依波加因抗性菌株中核糖体蛋白的改变。
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Foret1, a reverse transcriptase-like sequence in the filamentous fungus Fusarium oxysporum.Foret1,一种存在于丝状真菌尖孢镰刀菌中的类逆转录酶序列。
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Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.通过对S1核酸酶消化的杂交体进行凝胶电泳来确定早期腺病毒mRNA的大小并进行图谱分析。
Cell. 1977 Nov;12(3):721-32. doi: 10.1016/0092-8674(77)90272-0.