嗜鸟粪盘菌核糖体DNA重复单元的克隆与特性分析
Cloning and characterization of the rDNA repeat unit of Podospora anserina.
作者信息
Labat N, Perrot M, Bégueret J
出版信息
Mol Gen Genet. 1985;199(1):154-7. doi: 10.1007/BF00327526.
PMID:2987647
Abstract
DNA coding for ribosomal RNA in Podospora anserina has been cloned and was found as a tandemly repeated 8.3 kb sequence. The cloned rDNA was characterized by restriction endonuclease mapping. The location of 5.8S, 18S and 28S rRNA coding regions was established by DNA-RNA hybridization and S1 nuclease mapping. The organization of P. anserina rRNA genes is similar to that of Neurospora crassa and Aspergillus nidulans. The rDNA unit does not contain the sequence coding for 5S RNA.
摘要
已克隆了嗜鸟粪盘菌中编码核糖体RNA的DNA，发现其为一个串联重复的8.3 kb序列。通过限制性内切酶图谱分析对克隆的核糖体DNA进行了表征。通过DNA-RNA杂交和S1核酸酶图谱分析确定了5.8S、18S和28S核糖体RNA编码区的位置。嗜鸟粪盘菌核糖体RNA基因的组织方式与粗糙脉孢菌和构巢曲霉相似。核糖体DNA单元不包含编码5S RNA的序列。
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