Ten Leonid N, Lim Soo Jeong, Kim Byung-Oh, Kang In-Kyu, Jung Hee-Young
School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
School of Food Science and Biotechnology, Kyungpook National University, Daegu, 41566, Republic of Korea.
Arch Microbiol. 2018 Oct;200(8):1167-1175. doi: 10.1007/s00203-018-1536-9. Epub 2018 Jun 6.
A polyphasic taxonomic study was performed on a novel strain designated as S7-3-11, which was isolated from soil of the Gyeongsangnam-do province in Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain S7-3-11 belongs to the genus Hymenobacter and is most closely related to Hymenobacter ruber PB156 (97.9%), Hymenobacter daeguensis 16F3Y-2 (97.8%), Hymenobacter glaciei VUG-A130 (97.7%), Hymenobacter soli PB17 (97.5%), Hymenobacter terrae DG7A (97.5%), and Hymenobacter antarcticus VUG-A42aa (97.3%). However, DNA-DNA hybridization results showed less than 50% relatedness with respect to the type strains of the six most closely related species. The DNA G + C content of strain S7-3-11 was 60.2 mol%. MK-7 was identified as the predominant respiratory quinone, and summed feature 3 (C ω7c/C ω6c; 21.5%), C iso (16.8%), C anteiso (16.2%), and C iso G (10.8%) were the major fatty acids. Phosphatidylethanolamine, an unidentified aminolipid, and an unidentified aminophospholipid were detected as major polar lipids. On the basis of the polyphasic evidence presented, strain S7-3-11 is considered to represent a novel species of the genus Hymenobacter, for which the name Hymenobacter segetis sp. nov. is proposed. The type strain is S7-3-11 (= KCTC 52732 = JCM 32197).
对一株命名为S7-3-11的新菌株进行了多相分类研究，该菌株从大韩民国庆尚南道的土壤中分离得到。基于16S rRNA基因序列的系统发育分析表明，菌株S7-3-11属于鞘氨醇杆菌属，与红色鞘氨醇杆菌PB156（97.9%）、大邱鞘氨醇杆菌16F3Y-2（97.8%）、冰川鞘氨醇杆菌VUG-A130（97.7%）、土壤鞘氨醇杆菌PB17（97.5%）、土鞘氨醇杆菌DG7A（97.5%）和南极鞘氨醇杆菌VUG-A42aa（97.3%）关系最为密切。然而，DNA-DNA杂交结果显示，与六个关系最为密切的模式菌株的相关性均低于50%。菌株S7-3-11的DNA G+C含量为60.2 mol%。MK-7被鉴定为主要呼吸醌，特征性总和3（C ω7c/C ω6c；21.5%）、C异戊酸（16.8%）、Canteiso（16.2%）和C异戊酸G（10.8%）为主要脂肪酸。磷脂酰乙醇胺、一种未鉴定的氨基脂质和一种未鉴定的氨基磷脂被检测为主要极性脂质。基于所提供的多相证据，菌株S7-3-11被认为代表鞘氨醇杆菌属的一个新物种，为此提出了新名称土壤鞘氨醇杆菌（Hymenobacter segetis sp. nov.）。模式菌株为S7-3-11（=KCTC 52732=JCM 32197）。
