Lee Jae-Jin, Joo Eun Sun, Kim Eun Bit, Jeon Seon Hwa, Srinivasan Sathiyaraj, Jung Hee-Young, Kim Myung Kyum
Division of Environmntal & Life Science, Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul, 139-774, Republic of Korea.
College of Agricultural and Life Sciences, Kyungpook National University, Daegu, 702-701, Republic of Korea.
Antonie Van Leeuwenhoek. 2016 Mar;109(3):457-66. doi: 10.1007/s10482-016-0652-2. Epub 2016 Jan 25.
Strain DG7B(T) was isolated from a soil sample collected in Seoul, Republic of Korea and was observed to be a gram-negative, short-rod shaped and non-motile bacterium. Its 16S rRNA gene sequence is closely related to those of Hymenobacter terrae DG7A(T) (97.8 % similarity), H. soli PB17(T) (97.5 %), H. glaciei VUG-A130(T) (96.4 %), H. saemangeumensis GSR0100(T) (95.7 %), H. ruber PB156(T) (95.3 %), and H. antarcticus VUG-A42aa(T) (95.3 %). The low levels of DNA-DNA relatedness (<50.3 %) with the above species identified strain DG7B(T) as a novel species in the genus Hymenobacter. The genomic DNA G+C content was determined to be 54.9 %. Growth of strain DG7B(T) was observed at 12-30 °C (optimum at 25 °C) and pH 6.0-11.0 (optimum at pH 7). The cells tolerate <0.5 % NaCl. A UV-visible scan of an ethanol extract of the whole cell pigment showed absorbance peaks at 264.5, 320.0, and 481.5 nm, so the pigment type was determined to be 2'-hydroxyflexixanthin. Chemotaxonomic data showed that strain DG7B(T) possesses menaquinone-7 as the predominant isoprenoid quinone, sym-homospermidine as the major polyamine, phosphatidylethanolamine as the predominant polar lipid and iso-C15:0, anteiso-C15:0 and summed feature 3 (C16:1 ω7c/C16:1 ω7c) as the major fatty acids. Strain DG7B(T) showed low-level resistance to ultraviolet C. Based on the polyphasic analysis, it is concluded that strain DG7B(T) (=KCTC 32553(T) = KEMB 9004-166(T) = JCM 30008(T)) should be classified as the type strain of a novel Hymenobacter species, for which the name Hymenobacter rubidus sp. nov. is proposed.
菌株DG7B(T)是从韩国首尔采集的土壤样本中分离得到的，观察发现其为革兰氏阴性、短杆状且无运动性的细菌。其16S rRNA基因序列与土生嗜冻菌DG7A(T)（相似度97.8%）、土壤嗜冻菌PB17(T)（97.5%）、冰川嗜冻菌VUG-A130(T)（96.4%）、新万金海嗜冻菌GSR0100(T)（95.7%）、红色嗜冻菌PB156(T)（95.3%）以及南极嗜冻菌VUG-A42aa(T)（95.3%）的序列密切相关。与上述物种的DNA-DNA相关性较低（<50.3%），这表明菌株DG7B(T)是嗜冻菌属中的一个新物种。基因组DNA的G+C含量测定为54.9%。观察到菌株DG7B(T)在12 - 30 °C（最适温度25 °C）和pH 6.0 - 11.0（最适pH 7）条件下生长。细胞能耐受<0.5%的NaCl。对全细胞色素乙醇提取物的紫外可见扫描显示在264.5、320.0和481.5 nm处有吸收峰，因此确定色素类型为2'-羟基弯曲菌黄素。化学分类学数据表明，菌株DG7B(T)以甲基萘醌-7作为主要类异戊二烯醌，对称高亚精胺作为主要多胺，磷脂酰乙醇胺作为主要极性脂质，异-C15:0、anteiso-C15:0和总和特征3（C十六烷酸1 ω7c/C十六烷酸1 ω7c）作为主要脂肪酸。菌株DG7B(T)对紫外线C表现出低水平抗性。基于多相分析，得出结论：菌株DG7B(T)（=KCTC 32553(T) = KEMB 9004 - 166(T) = JCM 30008(T)）应被归类为嗜冻菌属一个新物种的模式菌株，为此提出新名称红色嗜冻菌（Hymenobacter rubidus sp. nov.）。
