Sompayrac L M, Danna K J
J Virol. 1982 Jul;43(1):328-31. doi: 10.1128/JVI.43.1.328-331.1982.
We constructed a tsB4/dl884 double-mutant helper virus and used it to isolate two simian virus 40 early region deletion mutants that lack about half of the DNA sequences normally used to encode the large tumor antigen (T). Both mutants make a normal-sized small t antigen, but neither mutant can replicate its DNA in the absence of a T+ helper.
我们构建了一种tsB4/dl884双突变辅助病毒，并利用它分离出两种猿猴病毒40早期区域缺失突变体，这些突变体缺少通常用于编码大T抗原（T）的大约一半DNA序列。两种突变体都能产生正常大小的小t抗原，但在没有T+辅助病毒的情况下，两种突变体都无法复制其DNA。
