猿猴病毒40早期区域缺失突变体的分离与鉴定
Isolation and characterization of simian virus 40 early region deletion mutants.
作者信息
Sompayrac L M, Danna K J
出版信息
J Virol. 1982 Jul;43(1):328-31. doi: 10.1128/JVI.43.1.328-331.1982.
Abstract
We constructed a tsB4/dl884 double-mutant helper virus and used it to isolate two simian virus 40 early region deletion mutants that lack about half of the DNA sequences normally used to encode the large tumor antigen (T). Both mutants make a normal-sized small t antigen, but neither mutant can replicate its DNA in the absence of a T+ helper.
摘要
我们构建了一种tsB4/dl884双突变辅助病毒，并利用它分离出两种猿猴病毒40早期区域缺失突变体，这些突变体缺少通常用于编码大T抗原（T）的大约一半DNA序列。两种突变体都能产生正常大小的小t抗原，但在没有T+辅助病毒的情况下，两种突变体都无法复制其DNA。
相似文献
1
Isolation and characterization of simian virus 40 early region deletion mutants.
J Virol. 1982 Jul;43(1):328-31. doi: 10.1128/JVI.43.1.328-331.1982.
2
Regulatory mutants of simian virus 40. Effect of mutations at a T antigen binding site on DNA replication and expression of viral genes.
J Mol Biol. 1982 Apr 15;156(3):531-48. doi: 10.1016/0022-2836(82)90265-0.
3
Construction and characterization of viable deletion mutants of simian virus 40 lacking sequences near the 3' end of the early region.
J Virol. 1982 Aug;43(2):489-502. doi: 10.1128/JVI.43.2.489-502.1982.
4
Mutational analysis of simian virus 40 T antigen: isolation and characterization of mutants with deletions in the T-antigen gene.
Mol Cell Biol. 1983 Feb;3(2):203-13. doi: 10.1128/mcb.3.2.203-213.1983.
5
A simian virus 40 dl884/tsA58 double mutant is temperature sensitive for abortive transformation.
J Virol. 1983 May;46(2):620-5. doi: 10.1128/JVI.46.2.620-625.1983.
6
Simian virus 40 mutant with transposed T-antigen and VP1 genes.
J Virol. 1982 Mar;41(3):1025-37. doi: 10.1128/JVI.41.3.1025-1037.1982.
7
Mutant carrying deletions in the two simian virus 40 early genes.
J Virol. 1981 Dec;40(3):625-34. doi: 10.1128/JVI.40.3.625-634.1981.
8
Nonviable mutants of simian virus 40 with deletions near the 3' end of gene A define a function for large T antigen required after onset of viral DNA replication.
J Virol. 1983 Sep;47(3):487-94. doi: 10.1128/JVI.47.3.487-494.1983.
9
Expression of early genes of origin-defective mutants of simian virus 40.
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3898-902. doi: 10.1073/pnas.77.7.3898.
10
Mutant of simian virus 40 large T-antigen that is defective for viral DNA synthesis, but competent for transformation of cultured rat cells.
J Virol. 1982 Jun;42(3):854-64. doi: 10.1128/JVI.42.3.854-864.1982.
引用本文的文献
1
Simian virus 40 deletion mutants that transform with reduced efficiency.
Mol Cell Biol. 1983 Mar;3(3):484-9. doi: 10.1128/mcb.3.3.484-489.1983.
2
A simian virus 40 dl884/tsA58 double mutant is temperature sensitive for abortive transformation.
J Virol. 1983 May;46(2):620-5. doi: 10.1128/JVI.46.2.620-625.1983.
3
Simian virus 40 sequences between 0.168 and 0.424 map units are not required for abortive transformation.
J Virol. 1983 May;46(2):475-80. doi: 10.1128/JVI.46.2.475-480.1983.
4
Stabilization of the 53,000-dalton nonviral tumor antigen is not required for transformation by simian virus 40.
Mol Cell Biol. 1983 Feb;3(2):290-6. doi: 10.1128/mcb.3.2.290-296.1983.
5
Less than 40% of the simian virus 40 large T-antigen-coding sequence is required for transformation.
Mol Cell Biol. 1984 Aug;4(8):1661-3. doi: 10.1128/mcb.4.8.1661-1663.1984.
6
The simian virus 40 sequences between 0.169 and 0.423 map units are not essential to immortalize early-passage rat embryo cells.
Mol Cell Biol. 1985 May;5(5):1191-4. doi: 10.1128/mcb.5.5.1191-1194.1985.
7
A fragment of the simian virus 40 early genome can induce tumors in nude mice.
J Virol. 1985 Mar;53(3):988-9. doi: 10.1128/JVI.53.3.988-989.1985.
8
A cis-acting sequence promotes removal of simian virus 40 DNA from the replication pool.
J Virol. 1985 Feb;53(2):410-4. doi: 10.1128/JVI.53.2.410-414.1985.
9
The T/t common region of simian virus 40 large T antigen contains a distinct transformation-governing sequence.
J Virol. 1991 Oct;65(10):5647-52. doi: 10.1128/JVI.65.10.5647-5652.1991.
本文引用的文献
1
Efficient infection of monkey cells with DNA of simian virus 40.
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7575-8. doi: 10.1073/pnas.78.12.7575.
2
Defective simian virus 40 genomes: isolation and growth of individual clones.
Virology. 1974 Nov;62(1):112-24. doi: 10.1016/0042-6822(74)90307-9.
3
Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon.
J Virol. 1972 Oct;10(4):591-8. doi: 10.1128/JVI.10.4.591-598.1972.
4
Inactivating and mutagenic effects of nitrosoguanidine on simian virus 40.
Proc Natl Acad Sci U S A. 1970 Jul;66(3):745-52. doi: 10.1073/pnas.66.3.745.
5
Enchancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran.
J Natl Cancer Inst. 1968 Aug;41(2):351-7.
6
Selective extraction of polyoma DNA from infected mouse cell cultures.
J Mol Biol. 1967 Jun 14;26(2):365-9. doi: 10.1016/0022-2836(67)90307-5.
7
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
8
A new method for sequencing DNA.
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4. doi: 10.1073/pnas.74.2.560.
9
Plasmids of Escherichia coli as cloning vectors.
Methods Enzymol. 1979;68:245-67. doi: 10.1016/0076-6879(79)68018-7.
10
Protein kinase activity associated with the avian sarcoma virus src gene product.
Proc Natl Acad Sci U S A. 1978 Apr;75(4):2021-4. doi: 10.1073/pnas.75.4.2021.
Zotero 插件