Department of Chemistry "G. Ciamician", University of Bologna, via Selmi, 2, Bologna, 40126, Italy.
Laboratory of Preclinical and Surgical Studies, IRCCS Rizzoli Orthopaedic Institute, via di Barbiano 1/10, 40136, Bologna, Italy.
Macromol Biosci. 2018 Jul;18(7):e1800096. doi: 10.1002/mabi.201800096. Epub 2018 Jun 7.
Strontium has a beneficial role on bone remodeling and is proposed for the treatment of pathologies associated to excessive bone resorption, such as osteoporosis. Herein, the possibility to utilize a biomimetic scaffold as strontium delivery system is explored. Porous 3D gelatin scaffolds containing about 30% of strontium substituted hydroxyapatite (SrHA) or pure hydroxyapatite (HA) are prepared by freeze-drying. The scaffolds display a very high open porosity, with an interconnectivity of 100%. Reinforcement with further amount of gelatin provokes a modest decrease of the average pore size, without reducing interconnectivity. Moreover, reinforced scaffolds display reduced water uptake ability and increased values of mechanical parameters when compared to as-prepared scaffolds. Strontium displays a sustained release in phosphate buffered saline: the quantities released after 14 d from as-prepared and reinforced scaffolds are just 14 and 18% of the initial content, respectively. Coculture of osteoblasts and osteoclasts shows that SrHA-containing scaffolds promote osteoblast viability and activity when compared to HA-containing scaffolds. On the other hand, osteoclastogenesis and osteoclast differentiation are significantly inhibited on SrHA-containing scaffolds, suggesting that these systems could be usefully applied for local delivery of strontium in loci characterized by excessive bone resorption.
锶对骨骼重塑具有有益作用，被提议用于治疗与过度骨吸收相关的病理学，如骨质疏松症。在此，探索了将仿生支架用作锶输送系统的可能性。通过冷冻干燥制备了含有约 30%锶取代羟基磷灰石（SrHA）或纯羟基磷灰石（HA）的多孔 3D 明胶支架。支架具有非常高的开孔率，连通性为 100%。进一步用明胶增强会适度降低平均孔径，而不会降低连通性。此外，与预制支架相比，增强的支架显示出吸水量减少和机械参数值增加的能力。锶在磷酸盐缓冲盐水中具有持续释放性：在 14 天内从预制和增强支架中释放的量分别仅为初始含量的 14%和 18%。成骨细胞和成骨细胞的共培养表明，与含有 HA 的支架相比，含有 SrHA 的支架可促进成骨细胞的活力和活性。另一方面，在含有 SrHA 的支架上，破骨细胞生成和分化被显著抑制，表明这些系统可用于在以过度骨吸收为特征的部位局部递送锶。
