Department of Mechanical Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
Biosens Bioelectron. 2018 Oct 15;117:15-22. doi: 10.1016/j.bios.2018.05.033. Epub 2018 May 22.
Direct extraction of photosynthetic electrons from the whole photosynthetic cells such as plant cells or algal cells can be highly efficient and sustainable compared to other approaches based on isolated photosynthetic apparatus such as photosystems I, II, and thylakoid membranes. However, insertion of nanoelectrodes (NEs) into individual cells are time-consuming and unsuitable for scale-up processes. We propose simple and efficient insertion of massively-populated NEs into cell films in which algal cells are densely packed in a monolayer. After stacking the cell film over an NE array, gentle pressing of the stack allows a large number of NEs to be inserted into the cells in the cell film. The NE array was fabricated by metal-assisted chemical etching (MAC-etching) followed by additional steps of wet oxidation and oxide etching. The cell film was prepared by mixing highly concentrated algal cells with alginate hydrogel. Photosynthetic currents of up to 106 nA/cm was achieved without aid of mediators, and the photosynthetic function was maintained for 6 days after NE array insertion into algal cells.
与基于分离的光合装置（如光系统 I、II 和类囊体膜）的其他方法相比，直接从植物细胞或藻类细胞等整个光合细胞中提取光合作用电子可以是高效和可持续的。然而，将纳米电极（NE）插入单个细胞既耗时又不适合大规模生产过程。我们提出了一种简单有效的方法，即将大量的 NE 插入到细胞薄膜中，其中藻类细胞在单层中密集排列。在将细胞薄膜堆叠在 NE 阵列上之后，轻轻按压堆栈可以将大量的 NE 插入到细胞薄膜中的细胞中。NE 阵列是通过金属辅助化学蚀刻（MAC-etching）制造的，然后进行额外的湿氧化和氧化物蚀刻步骤。细胞薄膜是通过将高浓度的藻类细胞与海藻酸钠水凝胶混合制备的。在没有介体的帮助下，实现了高达 106 nA/cm 的光合电流，并且在将 NE 阵列插入藻类细胞后，光合功能可以维持 6 天。
