染色体显带技术及其在栽培稻和野生稻中的应用。
Chromosome painting and its applications in cultivated and wild rice.
机构信息
Key Laboratory of Plant Functional Genomics of Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China.
Department of Horticulture, University of Wisconsin-Madison|, Madison, WI, 53706, USA.
出版信息
BMC Plant Biol. 2018 Jun 7;18(1):110. doi: 10.1186/s12870-018-1325-2.
BACKGROUND
The chromosome-specific probe is a fundamental tool of chromosome painting and has been commonly applied in mammalian species. The technology, however, has not been widely applied in plants due to a lack of methodologies for probe development. Identification and labeling of a large number of oligonucleotides (oligos) specific to a single chromosome offers us an opportunity to establish chromosome-specific probes in plants. However, never before has whole chromosome painting been performed in rice.
RESULTS
We developed a pooled chromosome 9-specific probe in rice, which contains 25,000 oligos based on the genome sequence of a japonica rice (Oryza sativa L., AA, 2n = 2× = 24). Chromosome 9 was easily identified in both japonica and indica rice using this chromosome 9-painting probe. The probe was also successfully used to identify and characterize chromosome 9 in additional lines of O. sativa, a translocation line, two new aneuploids associated with chromosome 9 and a wild rice (Oryza eichingeri A. Peter, CC, 2n = 2× = 24).
CONCLUSION
The study reveals that a pool of oligos specific to a chromosome is a useful tool for chromosome painting in rice.
背景
染色体特异性探针是染色体显色的基本工具，已广泛应用于哺乳动物物种。然而，由于缺乏探针开发方法，该技术尚未在植物中广泛应用。鉴定和标记大量针对单条染色体的寡核苷酸（oligos）为我们在植物中建立染色体特异性探针提供了机会。然而，以前从未在水稻中进行过整条染色体的显色。
结果
我们在水稻中开发了一种基于粳稻（Oryza sativa L.，AA，2n=2×=24）基因组序列的 9 号染色体特异性探针池，其中包含 25000 个 oligos。使用这种 9 号染色体显色探针，很容易在粳稻和籼稻中鉴定出 9 号染色体。该探针还成功地用于鉴定和表征水稻中的 9 号染色体、一个易位系、两个与 9 号染色体相关的新的非整倍体以及一种野生稻（Oryza eichingeri A. Peter，CC，2n=2×=24）。
结论
该研究表明，针对染色体的 oligos 池是水稻染色体显色的有用工具。
Zotero 插件