Schoeb T R, Kervin K C, Lindsey J R
Vet Pathol. 1985 May;22(3):272-82. doi: 10.1177/030098588502200310.
Six- to eight-week-old gnotobiotic F344/N rats were inoculated intranasally with 10(5.0) colony-forming units of Mycoplasma pulmonis or were sham inoculated, then one week later were given 10(0.2) 50% tissue culture infective doses of Sendai virus or sterile medium. Groups of rats were killed immediately after virus inoculation and three, five, ten, and 20 days later. Lesions in nasal passages, middle ears, larynxes, tracheas, and lungs from half of the rats in each group were subjectively scored. Organs from the other rats were quantitatively cultured for M. pulmonis and for Sendai virus. Rats given Sendai virus alone had mild, patchy, necrotizing rhinitis, laryngitis, tracheitis, and bronchitis, but not bronchiolitis or interstitial pneumonia. M. pulmonis alone induced mild lesions of murine respiratory mycoplasmosis including mild to moderate suppurative rhinitis, otitis media, laryngitis, and tracheitis with submucosal lymphoid accumulation and epithelial hyperplasia, but not lung lesions. Rats given M. pulmonis and Sendai virus had severe lesions characteristic of advanced mycoplasmal disease throughout the respiratory tract, including suppurative bronchitis with extensive lymphoid accumulations and epithelial hyperplasia; some rats also had suppurative pneumonia and bronchiectasis. Larger numbers of M. pulmonis colony-forming units were in rats given Sendai virus, but there was no statistically significant difference in Sendai virus infectious units between rats also given M. pulmonis and those given virus only.
将6至8周龄的无菌F344/N大鼠经鼻接种10(5.0)个肺炎支原体菌落形成单位，或进行假接种，然后在一周后给予10(0.2)个50%组织培养感染剂量的仙台病毒或无菌培养基。在病毒接种后立即以及接种后3天、5天、10天和20天处死大鼠组。对每组一半大鼠的鼻腔、中耳、喉、气管和肺中的病变进行主观评分。对另一半大鼠的器官进行肺炎支原体和仙台病毒的定量培养。仅给予仙台病毒的大鼠有轻度、散在性、坏死性鼻炎、喉炎、气管炎和支气管炎，但无细支气管炎或间质性肺炎。单独感染肺炎支原体引起轻度的鼠呼吸道支原体病病变，包括轻度至中度化脓性鼻炎、中耳炎、喉炎和气管炎，伴有粘膜下淋巴积聚和上皮增生，但无肺部病变。给予肺炎支原体和仙台病毒的大鼠在整个呼吸道有严重的晚期支原体病特征性病变，包括伴有广泛淋巴积聚和上皮增生的化脓性支气管炎；一些大鼠还患有化脓性肺炎和支气管扩张。给予仙台病毒的大鼠体内肺炎支原体菌落形成单位数量更多，但在同时给予肺炎支原体的大鼠和仅给予病毒的大鼠之间，仙台病毒感染单位无统计学显著差异。
