Pinson D M, Schoeb T R, Lindsey J R, Davis J K
Vet Pathol. 1986 Sep;23(5):550-5. doi: 10.1177/030098588602300502.
To evaluate lesions of ammonia exposure and Mycoplasma pulmonis infection in gnotobiotic F344/N rats, groups of rats were inoculated with M. pulmonis, exposed to 76 micrograms/liter (100 parts per million) ammonia, or both, or served as controls. Six rats from each group were killed 3, 5, 7, and 9 days after inoculation and their respiratory organs prepared for histologic examination. Lesions were assessed by subjective scoring and computerized morphometry, and results obtained by the two methods were compared to assess agreement of results. Lesions were limited to the nasal passages. Ammonia exposure resulted in hyperplastic and degenerative changes in the anterior nasal epithelium. Lesions of mycoplasmosis were more severe in ammonia-exposed than unexposed rats, but qualitative differences in lesions were not apparent. Results of the two methods agreed in assessment of submucosal inflammatory cell accumulation. Epithelial lesions were difficult to evaluate by both methods in infected, ammonia-exposed rats. In evaluation of lesions of ammonia exposure alone, agreement was adequate. Both methods indicated that exudates were significantly greater in ammonia-exposed infected rats than in non-exposed infected rats. Thus, scoring and morphometry provided similar assessments of differences in lesion severity among the treatment groups.
为评估悉生F344/N大鼠氨暴露和肺支原体感染的损伤情况，将大鼠分组，分别接种肺支原体、暴露于76微克/升（百万分之一百）的氨中、同时进行这两种处理或作为对照。每组6只大鼠在接种后3、5、7和9天处死，取其呼吸器官进行组织学检查。通过主观评分和计算机形态测定法评估损伤情况，并比较两种方法所得结果以评估结果的一致性。损伤仅限于鼻道。氨暴露导致鼻前部上皮细胞增生和退行性变化。与未暴露于氨的大鼠相比，暴露于氨的大鼠支原体病损伤更严重，但损伤的定性差异不明显。两种方法在评估黏膜下炎性细胞积聚方面结果一致。在感染且暴露于氨的大鼠中，两种方法都难以评估上皮损伤。在单独评估氨暴露损伤时，一致性良好。两种方法均表明，暴露于氨的感染大鼠的渗出物明显多于未暴露的感染大鼠。因此，评分和形态测定法对各治疗组损伤严重程度差异的评估结果相似。
