Laboratório de Biomarcadores, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário s/n, Pelotas, RS, Brazil.
Laboratório de Farmacognosia, Programa de Pós-Graduação em Biotecnologia e Biociências, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
Metab Brain Dis. 2018 Oct;33(5):1551-1562. doi: 10.1007/s11011-018-0262-y. Epub 2018 Jun 7.
In this work, we evaluated the effects of Psidium cattleianum (Red Type) (PcRT) fruit extract on metabolic, behavioral, and neurochemical parameters in rats fed with a highly palatable diet (HPD) consisted of sucrose (65% carbohydrates being 34% from condensed milk, 8% from sucrose and 23% from starch, 25% protein and 10% fat). Animals were divided into 4 groups: standard chow, standard chow + PcRT extract (200 mg/Kg/day by gavage), HPD, HPD + extract. The animals were treated for 150 days. Concerning chemical profiling, LC/PDA/MS/MS analysis revealed cyanidin-3-O-glucoside as the only anthocyanin in the PcRT extract. Our results showed that the animals exposed to HPD presented glucose intolerance, increased weight gain and visceral fat, as well as higher serum levels of glucose, triacylglycerol, total cholesterol, LDL-cholesterol and interleukin-6. These alterations were prevented by PcRT. In addition, HPD caused an increase in immobility time in a forced swimming test and the fruit extract prevented this alteration, indicating an antidepressant-like effect. PcRT treatment also prevented increased acetylcholinesterase activity in the prefrontal cortex caused by HPD consumption. Moreover, PcRT extract was able to restore Ca-ATPase activity in the prefrontal cortex, hippocampus, and striatum, as well as Na,K-ATPase activity in the prefrontal cortex and hippocampus. PcRT treatment decreased thiobarbituric acid-reactive substances, nitrite, and reactive oxygen species levels and prevented the reduction of superoxide dismutase activity in all cerebral structures of the HPD group. Additionally, HPD decreased catalase in the hippocampus and striatum. However, the extract prevented this change in the hippocampus. Our results showed that this berry extract has antihyperglycemic and antihyperlipidemic effects, and neuroprotective properties, proving to be a potential therapeutic agent for individuals with metabolic syndrome.
在这项工作中，我们评估了 Psidium cattleianum（红色品种）（PcRT）果实提取物对喂食高可口饮食（HPD）的大鼠的代谢、行为和神经化学参数的影响。HPD 由蔗糖（65%碳水化合物，其中 34%来自炼乳，8%来自蔗糖，23%来自淀粉）、25%蛋白质和 10%脂肪组成。动物分为 4 组：标准饲料、标准饲料+PcRT 提取物（通过灌胃每天 200mg/kg）、HPD、HPD+提取物。动物接受治疗 150 天。关于化学特征分析，LC/PDA/MS/MS 分析显示，PcRT 提取物中只有矢车菊素-3-O-葡萄糖苷作为花色苷。我们的结果表明，暴露于 HPD 的动物表现出葡萄糖不耐受、体重增加和内脏脂肪增加，以及血清中葡萄糖、三酰甘油、总胆固醇、LDL-胆固醇和白细胞介素-6 水平升高。PcRT 可预防这些变化。此外，HPD 导致强迫游泳试验中不动时间增加，而果实提取物可预防这种变化，表明具有抗抑郁样作用。PcRT 处理还可预防 HPD 消耗引起的前额叶皮层乙酰胆碱酯酶活性增加。此外，PcRT 提取物能够恢复 HPD 组前额叶皮层、海马和纹状体的 Ca-ATPase 活性，以及前额叶皮层和海马的 Na，K-ATPase 活性。PcRT 处理降低了所有脑结构中丙二醛反应产物、亚硝酸盐和活性氧物质的水平，并防止了 HPD 组中超氧化物歧化酶活性的降低。此外，HPD 降低了海马和纹状体中的过氧化氢酶。然而，提取物防止了这种在海马体中的变化。我们的结果表明，这种浆果提取物具有降血糖和降血脂作用，以及神经保护特性，可作为代谢综合征个体的潜在治疗剂。
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