Oliveira Pathise Souto, Chaves Vitor Clasen, Bona Natália Pontes, Soares Mayara Sandrielly Pereira, Cardoso Juliane de Souza, Vasconcellos Flávia Aleixo, Tavares Rejane Giacomelli, Vizzotto Marcia, Silva Luísa Mariano Cerqueira da, Grecco Fabiane Borelli, Gamaro Giovana Duzzo, Spanevello Roselia Maria, Lencina Claiton Leoneti, Reginatto Flávio Henrique, Stefanello Francieli Moro
Laboratório de Biomarcadores, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário s/n, Pelotas, RS, Brazil.
Laboratório de Farmacognosia, Programa de Pós-Graduação em Biotecnologia e Biociências, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
Biomed Pharmacother. 2017 Aug;92:935-941. doi: 10.1016/j.biopha.2017.05.131. Epub 2017 Jun 10.
The aim of this study was to investigate the effect of Eugenia uniflora fruit (red type) extract on metabolic status, as well as on neurochemical and behavioral parameters in an animal model of metabolic syndrome induced by a highly palatable diet (HPD). Rats were treated for 150days and divided into 4 experimental groups: standard chow (SC) and water orally, SC and E. uniflora extract (200mg/kg daily, p.o), HPD and water orally, HPD and extract. Our data showed that HPD caused glucose intolerance, increased visceral fat, weight gain, as well as serum glucose, triacylglycerol, total cholesterol and LDL cholesterol; however, E. uniflora prevented these alterations. The extract decreased lipid peroxidation and prevented the reduction of superoxide dismutase and catalase activities in the prefrontal cortex, hippocampus and striatum of animals submitted to HPD. We observed a HPD-induced reduction of thiol content in these cerebral structures. The extract prevented increased acetylcholinesterase activity in the prefrontal cortex caused by HPD and the increase in immobility time observed in the forced swim test. Regarding chemical composition, LC/MS analysis showed the presence of nine anthocyanins as the major compounds. In conclusion, E. uniflora extract showed benefits against metabolic alterations caused by HPD, as well as exhibited antioxidant and antidepressant-like effects.
本研究的目的是调查番樱桃（红色品种）果实提取物对代谢状态的影响，以及对由高糖高脂饮食（HPD）诱导的代谢综合征动物模型的神经化学和行为参数的影响。大鼠接受150天的治疗，并分为4个实验组：口服标准饲料（SC）和水、口服SC和番樱桃提取物（每日200mg/kg，口服）、口服HPD和水、口服HPD和提取物。我们的数据显示，HPD导致葡萄糖不耐受、内脏脂肪增加、体重增加，以及血清葡萄糖、三酰甘油、总胆固醇和低密度脂蛋白胆固醇升高；然而，番樱桃提取物可预防这些改变。提取物可降低脂质过氧化，并防止HPD诱导的动物前额叶皮质、海马体和纹状体中超氧化物歧化酶和过氧化氢酶活性的降低。我们观察到HPD导致这些脑区结构中硫醇含量降低。提取物可预防HPD引起的前额叶皮质中乙酰胆碱酯酶活性升高，以及强迫游泳试验中不动时间的增加。关于化学成分，液相色谱/质谱分析显示存在9种花青素作为主要化合物。总之，番樱桃提取物对HPD引起的代谢改变具有有益作用，并且具有抗氧化和抗抑郁样作用。
