硅纳米颗粒通过与间充质干细胞的相互作用来改善急性功能心脏整合。
Silica nanoparticles actively engage with mesenchymal stem cells in improving acute functional cardiac integration.
机构信息
Department of Clinical & Biological Sciences, University of Turin, Regione Gonzole 10, 10043 Orbassano, Turin, Italy.
Department of Chemistry, Interdepartmental Centre "Nanostructured Interfaces & Surfaces" University of Turin, Via P. Giuria 7, 10125 Turin, Italy.
出版信息
Nanomedicine (Lond). 2018 May;13(10):1121-1138. doi: 10.2217/nnm-2017-0309. Epub 2018 Jun 8.
AIM
To assess functional effects of silica nanoparticles (SiO-NPs) on human mesenchymal stem cell (hMSC) cardiac integration potential.
METHODS
SiO-NPs were synthesized and their internalization effects on hMSCs analyzed with particular emphasis on interaction of hMSCs with the cardiac environment Results: SiO-NP internalization affected the area and maturation level of hMSC focal adhesions, accounting for increased in vitro adhesion capacity and augmented engraftment in the myocardial tissue upon cell injection in infarcted isolated rat hearts. SiO-NP treatment also enhanced hMSC expression of Connexin-43, favoring hMSC interaction with cocultured cardiac myoblasts in an ischemia-like environment.
CONCLUSION
These findings provide strong evidence that SiO-NPs actively engage in mediating biological effects, ultimately resulting in augmented hMSC acute cardiac integration potential.
目的
评估二氧化硅纳米颗粒（SiO-NPs）对人骨髓间充质干细胞（hMSC）心脏整合潜能的功能影响。
方法
合成 SiO-NPs 并分析其对 hMSC 的内化作用，特别强调 hMSC 与心脏环境的相互作用。
结果
SiO-NP 的内化影响 hMSC 焦点黏附的面积和成熟度水平，导致体外黏附能力增加，并在细胞注射到缺血性孤立大鼠心脏的梗死部位后增强了植入。SiO-NP 处理还增强了 hMSC 连接蛋白 43 的表达，有利于 hMSC 在类似缺血的环境中与共培养的心肌细胞相互作用。
结论
这些发现有力地证明了 SiO-NPs 积极参与介导生物学效应，最终导致 hMSC 急性心脏整合潜能增强。
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